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Characterization of deoxynivalenol dehydrogenase from Pelagibacterium sp. SCN 63-126 and its application. | LitMetric

Characterization of deoxynivalenol dehydrogenase from Pelagibacterium sp. SCN 63-126 and its application.

Arch Microbiol

State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi, Jiangsu, 214122, P. R. China.

Published: December 2024

AI Article Synopsis

  • Deoxynivalenol (DON) is a harmful mycotoxin produced by Fusarium species, commonly found in wheat and grains, and has been a focus for enzymatic degradation studies.
  • The study identifies a dehydrogenase enzyme (Pe DDH) from Pelagibacterium sp. SCN 63-126 that effectively breaks down DON, demonstrating optimal activity at 35°C and pH 8.5.
  • Pe DDH's activity is significantly enhanced by the presence of calcium, magnesium, copper, and certain chemical cofactors, allowing it to degrade DON quickly, highlighting its potential for reducing DON contamination in grains.*

Article Abstract

Deoxynivalenol (DON), a type-B trichothecene mycotoxin, is primarily produced by Fusarium species and widely pollutes wheat and other grains. Enzymatic treatment of DON has been widely studied in recent years. Here, we present the biochemical identification of the DON dehydrogenase from Pelagibacterium sp. SCN 63-126 (Pe DDH). After removing the signal peptide, Pe DDH is effectively expressed in its soluble form. Biochemical identification indicates that the optimal temperature and pH of Pe DDH against DON is 35 ℃ and pH 8.5. Furthermore, Pe DDH is activated significantly in the presence of Ca, Mg, and Cu, and alternatively activated by pyrroloquinoline quinone (PQQ), phenazine methosulfate (PMS), and 2, 6-dichlorophenolindophenol (DCPIP). When PQQ, PMS, and DCPIP are combined, Pe DDH (60 µg/mL) effectively degrads DON (150 µM) in just 5 min, suggesting a synergistic effect of three cofactors on DON degradation. All these results suggest a great potential of Pe DDH in the control of DON contamination.

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Source
http://dx.doi.org/10.1007/s00203-024-04208-9DOI Listing

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