Utilizing photosynthetic oxygen-releasing biomaterials to modulate blood vessel growth in the chick embryo chorioallantoic membrane.

Effective vascularization is crucial for the success of tissue engineering and is influenced by numerous factors. The present work focuses on investigating the effect of a substance, cyanobacteria-loaded oxygen-releasing hydrogel, on vascularization and verifying the effect of photosynthetic-oxygen-releasing biomaterials containing a cyanobacteria hydrogel on angiogenesis, using the chick chorioallantoic membrane (CAM) as a model system. On the eighth day of embryonic development, cyanobacterial microspheres were placed on the CAM and maintained in a light incubator under appropriate growth and photosynthesis conditions. The effect of cyanobacterial microspheres on vascularization was evaluated from the eighth day of embryonic development. The carrier material used to prepare the microspheres was a calcium alginate hydrogel, which is biocompatible for maintaining embryonic vitality. The article studied the preparation method, the optimal process, and the specific effects of co-culture on CAM vascularization and development. The data indicate that our prepared photosynthetic oxygen-releasing blue-green algal microspheres have the potential for symbiosis with tissues by supplying oxygen to tissues and inducing vascular growth through photosynthetic oxygen release. This research opens new avenues for applying cyanobacterial microspheres, a novel biological oxygen-releasing material, in regenerative medicine.