Lysosomal biothiols play critical roles in numerous cellular processes and diseases. Researching an effective method for real-time labeling biothiols in lysosomes is of great significance and urgency, as it could provide essential information for the diagnosis of relevant diseases. In this study, we developed a lysosome-targeted fluorescent probe (LY-DCM-P) with a large Stokes shift of 150 nm for the sensitive and selective detection of biothiols and . Additionally, LY-DCM-P showed low cytotoxicity and excellent lysosome-targeted ability. The probe was successfully employed to monitor fluctuations in lysosomal biothiols in various living systems, enabling enormous potential to accurately monitor the occurrence and progress of biothiol-related diseases.
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| http://dx.doi.org/10.1016/j.isci.2024.111334 | DOI Listing |
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