AI Article Synopsis

  • Human African trypanosomiasis (HAT) is a serious disease that affects both humans and animals, causing severe health issues, and this study explores the medicinal properties of plant extracts used to treat it.
  • The ethanol extracts from fruit (CFE), neem leaves (NLE), and stem bark (NSBE) showed significant antitrypanosomal activity, with CFE being the most effective in inhibiting the parasite, while all extracts were non-toxic to liver cells.
  • Despite NSBE having the highest antioxidant and anti-inflammatory properties, the study found that CFE and NLE have different mechanisms leading to their superior antitrypanosomal effects, leading to ongoing research on CFE’s constituents for potential new treatments

Article Abstract

Human African trypanosomiasis (HAT) is an infectious disease which kills humans and animals as a result of hematological distortions, oxidative stress, tissue and neuroinflammations. This study reports on the differences in cytotoxicity, antitrypanosomal, antioxidant, and anti-inflammatory activities of ethanol extracts from fruit (CFE), (neem) leaf (NLE), and stem bark (NSBE), medicinal plants used to treat HAT in its endemic communities. The extracts remarkably inhibited (GUTat 3.1) parasite in vitro with CFE recording the highest effect with an IC of 0.0055 (0.0955) g/mL. The IC of the standard was 0.5957 (0.0693) g/mL. Also, the antitrypanosomal activity of NLE was 123.34% higher than that of NSBE. The percentage number of wells containing viable parasites was very significantly ( < 0.001) reduced for all the extracts after 48 h of incubation. Furthermore, the extracts did not show cytotoxicity against the liver (HepG2) cells (CCs > 100 g/mL and SI = 13.12-32,025.45). NSBE contained the highest quantity of phenolic compounds and flavonoids and also produced the highest antioxidant and anti-inflammatory activity in the DPPH free radical scavenging assay (IC = 4.99 ± 0.018) and protein denaturation assay (IC = 0.1805 ± 0.0002 g/mL). In addition, phytochemical analysis showed that NLE contained the highest number of classes of phytochemical constituents (seven) among the extracts. These results indicate that CFE, NLE, and NSBE possessed significant antitrypanosomal activity as a result of their antioxidant and anti-inflammatory actions. However, a different mechanism was also involved in the antitrypanosomal activity of CFE and NLE, since their antitrypanosomal activity is greater than NSBE which demonstrated the highest antioxidant and anti-inflammatory activities. Due to the remarkable antitrypanosomal action of CFE, its constituents are being isolated for possible development into novel antitrypanosomal agents.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11617053PMC
http://dx.doi.org/10.1155/japr/7509588DOI Listing

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