AI Article Synopsis

  • Traumatic spinal cord injury (SCI) leads to permanent loss of movement and sensation below the injury, making effective therapies a complex challenge despite ongoing research.
  • Our lab created a new therapy using human adipose-derived stem cells within hydrogels made from different ratios of decellularized sciatic nerve and spinal cord materials, aiming to promote repair.
  • The study found that the composition of the hydrogels and the duration of culture influenced the release of growth factors, but different hydrogel formulations did not significantly alter certain cell behaviors, with the 1:2 ratio of nerve to spinal cord showing the most potential for further research.

Article Abstract

Traumatic spinal cord injury (SCI) induces permanent sensorimotor deficit below the site of injury. There is various research conducted to provide effective therapy, however, SCI is still considered incurable due to the complex nature of the injury site. Recently, our lab developed a combinatorial therapeutic for SCI repair comprising human adipose-derived stem cell (hASC)-embedded nerve composite hydrogels using different ratios of decellularized sciatic nerve (dSN) and spinal cord (dSC) matrices. This study investigated angiogenic and neurotrophic effects of the combinatorial therapeutic in vitro. Compression testing was performed to analyze mechanical properties of the composite hydrogels and showed no significant difference between all hydrogel groups. Next, pro-angiogenic factors and neurotrophins secreted from hASCs within different ratios of the composite hydrogels were analyzed and we found culture durations and extracellular matrix (ECM) composition affect secretory behavior. Interestingly, ECM compositional difference between hydrogel groups had little influence on human brain microvascular endothelial cells (HBVECs) infiltration and dorsal root ganglia (DRG) neurite outgrowth. Finally, we conducted proteomic analysis to identify the ECM components potentially contributing to these observed effects. Taken together, dSN:dSC = 1:2 hydrogel showed slightly better therapeutic potentials, warranting validation using studies.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11616072PMC
http://dx.doi.org/10.1016/j.mbplus.2024.100165DOI Listing

Publication Analysis

Top Keywords

composite hydrogels
12
nerve composite
8
adipose-derived stem
8
spinal cord
8
combinatorial therapeutic
8
difference hydrogel
8
hydrogel groups
8
investigation neuro-regenerative
4
therapeutic
4
neuro-regenerative therapeutic
4

Similar Publications

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!