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Application of metagenomic next-generation sequencing (mNGS) to describe the microbial characteristics of diabetic foot ulcers at a tertiary medical center in South China.
BMC Endocr Disord
January 2025
Burn and Wound Repair Department, Fujian Medical University Union Hospital, Fuzhou, China.
Background: Diabetic foot ulcers (DFUs) are characterized by dynamic wound microbiome, the timely and accurate identification of pathogens in the clinic is required to initiate precise and individualized treatment. Metagenomic next-generation sequencing (mNGS) has been a useful supplement to routine culture method for the etiological diagnosis of DFUs. In this study, we utilized a routine culture method and mNGS to analyze the same DFU wound samples and the results were compared.
View Article and Find Full Text PDFA Balamuthia amoebic encephalitis survivor in China, and literature review.
Diagn Microbiol Infect Dis
January 2025
Department of pathology, Afficiated Hospital of Hebei University, Hebei, Baoding, 071000, China.. Electronic address:
Balamuthia amoebic encephalitis (BAE) is a rare, fatal parasitic infection of the central nervous system, with a current mortality rate above 95%. The high fatality rate is largely attributed to atypical clinicopathological features, delayed diagnosis, and the absence of effective treatment methods, so quick recognition of this disease is vital. In this paper, we present a survivor of BAE, who was confirmed through histologic examination and metagenomic next-generation sequencing (mNGS) of brain lesions.
View Article and Find Full Text PDFApplication of metagenomic next-generation sequencing in the diagnosis of post-stroke infections: a case series study using multiple sample types.
Front Cell Infect Microbiol
January 2025
Department of Neurology, The First Affiliated Hospital of Shantou University Medical College, Shantou, Guangdong, China.
Background: Metagenomic high-throughput sequencing (mNGS) represents a powerful tool for detecting nucleic acids from various pathogens, such as bacteria, fungi, viruses and parasites, in clinical samples. Despite its extensively employed in the pathogen diagnosis for various infectious diseases, its application in diagnosing stroke-related infection, and its potential impact on clinical decision-making, anti-infection treatment, clinical intervention, and patient prognosis remain insufficiently explored. Additionally, while mNGS offers promising potential, it facts limitations related to sensitivity, specificity, cost, and standardization, which could influence its integration into routine clinical practice.
View Article and Find Full Text PDFAn updated reference genome of Barbatula barbatula (Linnaeus, 1758).
Sci Data
January 2025
HUN-REN Institite of Aquatic Ecology, Centre for Ecological Research, Budapest, Hungary.
The stone loach Barbatula barbatula is a benthic fish species widely distributed throughout Europe, primarily inhabiting stony upper sections of stream networks. This study presents an updated genome assembly of B. barbatula, contributing to the species' available genomic resources for downstream applications such as conservation genetics.
View Article and Find Full Text PDFExploring animal food microbiomes and resistomes via 16S rRNA gene amplicon sequencing and shotgun metagenomics.
Appl Environ Microbiol
January 2025
Office of Applied Science, Center for Veterinary Medicine, U.S. Food and Drug Administration, Laurel, Maryland, USA.
As a diverse and complex food matrix, the animal food microbiota and repertoire of antimicrobial resistance (AMR) genes remain to be better understood. In this study, 16S rRNA gene amplicon sequencing and shotgun metagenomics were applied to three types of animal food samples (cattle feed, dry dog food, and poultry feed). ZymoBIOMICS mock microbial community was used for workflow optimization including DNA extraction kits and bead-beating conditions.
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