AI Article Synopsis

  • - This study explores how fucoidan, a natural polysaccharide, works alone and with antibiotics against biofilms formed by Methicillin-resistant Staphylococcus aureus (MRSA) and Acinetobacter baumannii, particularly in cases related to diabetic foot ulcers (DFUs).
  • - The results showed that using fucoidan with antibiotics like gentamicin and imipenem significantly enhances antimicrobial effectiveness, especially in mixed-species biofilms, with low toxicity to human skin cells.
  • - The findings suggest that fucoidan could be a promising treatment to disrupt harmful biofilms in DFU patients, indicating the need for more clinical research in this area.

Article Abstract

Introduction This study investigates the efficacy of fucoidan, both alone and in combination with antibiotics, against single-species biofilms and mixed-species, individual planktonic, and co-culture planktonic conditions of Methicillin-resistant Staphylococcus aureus (MRSA) and Acinetobacter baumannii. Materials and Methods Fucoidan, a sulfated polysaccharide, was purchased from Sigma-Aldrich, USA. Clinical isolates of MRSA and A. baumannii were obtained from diabetic foot ulcers (DFUs), and single-species biofilms and mixed-species biofilms were developed to assess susceptibility to the treatments using MIC, MBC, MBIC, MBEC, and time-kill kinetics assays. Cytotoxicity was assessed using MTT assays on human skin fibroblast cells (HSF-PI 16). Results The study determined the geometric mean MIC and MBC values for gentamicin, imipenem, and fucoidan in MRSA and A. baumannii cultures, both individually and in co-culture. The MIC and MBC values were significantly lower under co-culture conditions, indicating enhanced antimicrobial efficacy. Synergy between fucoidan, gentamicin, and imipenem was confirmed through time-kill assays, which showed complete inhibition of bacterial growth and effective biofilm eradication, particularly in mixed-species biofilms. Fucoidan demonstrated low cytotoxicity at optimal concentrations, highlighting their potential as a therapeutic strategy against biofilm-associated infections in diabetic foot ulcers. Conclusion The study concludes that fucoidan, in combination with gentamicin and imipenem, effectively disrupts mixed-species biofilms of MRSA and A. baumannii, suggesting fucoidan-based therapies could improve outcomes for DFU patients, warranting further clinical investigation.

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Source
http://dx.doi.org/10.1159/000542826DOI Listing

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