AI Article Synopsis
- β-glucosidases are essential for cellulase production in fungi, but their specific molecular functions and mechanisms are still unclear.
- Researchers found that among 10 studied β-glucosidases, one was vital for cellulase production and identified various affected biological processes in a deficient strain, like cellulase synthesis and ribosome biogenesis.
- The unconventional transport of CEL3J and its secretion pathway were linked to the mTORC1-GRASP55 signaling axis, suggesting new insights into the role of β-glucosidases and the mechanisms behind their secretion in fungi.
Article Abstract
β-glucosidases play an important role in the synthesis of cellulase in fungi, but their molecular functions and mechanisms remain unknown. We found that the 10 putative β-glucosidases investigated in facilitate cellulase production, with being the most crucial Transcriptional analysis revealed that the most affected biological processes in △ strain were cellulase synthesis, ribosome biogenesis, and RNA polymerases. Moreover, CEL3J was unconventionally transported through the endoplasmic reticulum, bypassing the Golgi apparatus, whereas overexpression altered cellulase secretion from conventional to unconventional, likely owing to the activated unconventional protein secretion pathway (UPS), as indicated by the upregulation of genes related to UPS. The mTORC1-GRASP55 signaling axis may modulate the unconventional secretion of CEL3J and cellulase. The transcriptional levels of genes associated with DNA replication, the cell cycle, and meiosis were noticeably affected by overexpressing . These data give new clues for exploring the roles of β-glucosidases and the molecular mechanisms of their unconventional secretion in fungi.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11610954 | PMC |
| http://dx.doi.org/10.1016/j.engmic.2023.100105 | DOI Listing |
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