characterization experiments revealed the formations of 3-(-2'-aminocyclopropyl)alanine ((3-Acp)Ala) and 3-(-2'-nitrocyclopropyl)alanine ((3-Ncp)Ala) are originated via two homologous proteins, BelK and HrmI, which regioselectively catalyze the Nε-oxygenation of l-lysine. The two enzymes belong to the emerging heme-oxygenase-like diiron oxidase and oxygenase (HDO) superfamily and the catalytic center of BelK is validated by homology modeling and site-directed mutations. Based on the characterization, the biosynthetic pathways of (3-Acp)Ala and (3-Ncp)Ala are proposed.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11611017 | PMC |
| http://dx.doi.org/10.1016/j.engmic.2021.100007 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
characterization of a nitro-forming oxygenase involved in 3-(-2'-aminocyclopropyl)alanine biosynthesis.
Eng Microbiol
March 2022
Helmholtz International Lab for AntiInfectives, Shandong University-Helmholtz Institute of Biotechnology, State Key Laboratory of Microbial Technology, Shandong University, Qingdao 266237, China.
characterization experiments revealed the formations of 3-(-2'-aminocyclopropyl)alanine ((3-Acp)Ala) and 3-(-2'-nitrocyclopropyl)alanine ((3-Ncp)Ala) are originated via two homologous proteins, BelK and HrmI, which regioselectively catalyze the Nε-oxygenation of l-lysine. The two enzymes belong to the emerging heme-oxygenase-like diiron oxidase and oxygenase (HDO) superfamily and the catalytic center of BelK is validated by homology modeling and site-directed mutations. Based on the characterization, the biosynthetic pathways of (3-Acp)Ala and (3-Ncp)Ala are proposed.
View Article and Find Full Text PDFCharacterization of a Nitro-Forming Enzyme Involved in Fosfazinomycin Biosynthesis.
Biochemistry
September 2021
Department of Biochemistry, Virginia Tech, Blacksburg, Virginia 24061, United States.
-hydroxylating monooxygenases (NMOs) are a subclass of flavin-dependent enzymes that hydroxylate nitrogen atoms. Recently, unique NMOs that perform multiple reactions on one substrate molecule have been identified. Fosfazinomycin M (FzmM) is one such NMO, forming nitrosuccinate from aspartate (Asp) in the fosfazinomycin biosynthetic pathway in some sp.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!