AI Article Synopsis
Article Abstract
Aims: Visceral adipose tissue (VAT) accumulation is essential for the occurrence and development of obesity and related metabolic diseases. Currently, the specific mechanism of VAT accumulation is still unclear.
Materials And Methods: We searched the Gene Expression Omnibus database to obtain single-cell RNA sequencing (scRNAseq) data for VAT in patients with a normal body mass index (BMI), obesity, or morbid obesity. By using PCR, WB, immunofluorescence staining, and flow cytometry analysis, we validated the interactions between macrophages, endothelial cells (ECs), and adipocyte progenitor cells (APCs), as well as the underlying mechanism, in VAT. Finally, we tested the findings in obese mice using recombinant proteins and adeno-associated virus infection.
Results: One study with human scRNAseq data was included. This study collected 13-VAT from 5 individuals with obesity and diabetes, 9 individuals with obesity, and 1 individual with a normal BMI. The proportion of inflammatory macrophages is substantially increased in obese and diabetic patients. ECs have the most active interactions with other cells. Notably, the activation of JAK1/STAT3 is one of the reasons for the increase in inflammatory endothelial cells and can promote the secretion of angiopoietin-2 (Angpt2) and induce APCs to transition from mesothelin (MSLN) to complement factor D (CFD) expression via integrin-α5β1 signalling. This phenotypic transition promotes APC differentiation into mature adipocytes and accelerates VAT accumulation. These observations were further validated in an in vitro model and an in vivo study using Angpt2 recombinant proteins and blocking the expression of Angpt2 by adeno-associated virus infection.
Conclusions: ECs are essential for promoting VAT accumulation by facilitating APC differentiation from MSLN to CFD phenotype. This process is driven by Angpt2 from ECs upon JAK1/STAT3 signalling activation under metabolic stress.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1002/dmrr.70012 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Associations of renal sinus fat with metabolic parameters, abdominal visceral adipose tissue, metabolic syndrome, fructose intake, and blood pressure control in obese individuals with hypertension: a cross-sectional study.
J Nutr Sci
December 2024
Kidney Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Renal sinus fat (RSF) crucially influences metabolic regulation, inflammation, and vascular function. We investigated the association between RSF accumulation, metabolic disorders, and nutritional status in obese individuals with hypertension. A cross-sectional study involved 51 obese hypertensive patients from Salamat Specialized Community Clinic (February-September 2022).
View Article and Find Full Text PDFEndothelial Angpt2 Promotes Adipocyte Progenitor Cells Maturation to Increase Visceral Adipose Tissue Accumulation.
Diabetes Metab Res Rev
January 2025
Department of General Surgery, The Third Xiangya Hospital, Central South University, Changsha, China.
Article Synopsis
Identification of metabolic syndrome using lipid accumulation product and cardiometabolic index based on NHANES data from 2005 to 2018.
Nutr Metab (Lond)
November 2024
Department of Cardiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China.
Background: Numerous studies indicate that visceral adipose tissue (VAT) significantly contribute to metabolic syndrome (MetS) development. This study aims to assess the distinguishing value of novel obesity markers, specifically lipid accumulation products (LAP) and cardiometabolic index (CMI), in relation to MetS. Considering the gender disparity in MetS prevalence, it is essential to explore whether LAP and CMI exhibit differential distinguishing capabilities by gender.
View Article and Find Full Text PDFVisceral adipose tissue, epicardial fat, and hepatic steatosis in polycystic ovary syndrome: a study of ectopic fat stores and metabolic dysfunction.
Endocrine
October 2024
Postgraduate Program in Clinical and Experimental Pathophysiology, Faculty of Medical Sciences, State University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
Purpose: In polycystic ovary syndrome (PCOS), ectopic fat accumulation remains debatable. Therefore, intra-abdominal, hepatic, and epicardial fat were compared between PCOS women and body mass index (BMI)-matched controls and their associations with metabolic and hormonal parameters were explored. Furthermore, the performance of echocardiographic epicardial adipose tissue thickness (EATT) and hepatic steatosis measurement using transient elastography-based controlled attenuation parameter (TE-CAP) in screening abdominal visceral adipose tissue (VAT) was originally evaluated.
View Article and Find Full Text PDFA long-term high-fat diet induces differential gene expression changes in spatially distinct adipose tissue of male mice.
Physiol Genomics
December 2024
Department of Biological Sciences, College of Arts and Sciences, University of Delaware, Newark, Delaware, United States.
Article Synopsis
- The study shows that having too much visceral fat (VAT) is linked to heart disease and diabetes, while having more subcutaneous fat (SAT) might not be as bad if VAT is not high.
- Scientists fed mice a high-fat diet to see how obesity affects specific fat types and found that obesity changes the way fat genes work.
- They discovered that certain genes behave differently in SAT and VAT when mice are obese, and these genes could help us understand more about fat and health problems.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!