AI Article Synopsis
- The mammalian pancreas has three key parts: exocrine acini and ducts, along with endocrine islets, all originating from a common progenitor during development.
- Researchers created 18 human fetal pancreas organoid lines from samples between 8-17 weeks of gestation, with four lines showing the ability to produce all three cell types while thriving in culture for over two years.
- Single-cell RNA sequencing revealed LGR5 cells as crucial developmental stem cells, indicating that these organoids are capable of long-term growth and can differentiate into acinar, ductal, and endocrine cells.
Article Abstract
The mammalian pancreas consists of three epithelial compartments: the acini and ducts of the exocrine pancreas and the endocrine islets of Langerhans. Murine studies indicate that these three compartments derive from a transient, common pancreatic progenitor. Here, we report derivation of 18 human fetal pancreas organoid (hfPO) lines from gestational weeks 8-17 (8-17 GWs) fetal pancreas samples. Four of these lines, derived from 15 to 16 GWs samples, generate acinar-, ductal-, and endocrine-lineage cells while expanding exponentially for >2 years under optimized culture conditions. Single-cell RNA sequencing identifies rare LGR5 cells in fetal pancreas and in hfPOs as the root of the developmental hierarchy. These LGR5 cells share multiple markers with adult gastrointestinal tract stem cells. Organoids derived from single LGR5 organoid-derived cells recapitulate this tripotency in vitro. We describe a human fetal tripotent stem/progenitor cell capable of long-term expansion in vitro and of generating all three pancreatic cell lineages.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.cell.2024.10.044 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!