AI Article Synopsis
- - Pharmaceutical wastewater treatment plants (PWWTPs) can harbor antibiotic-resistant bacteria (ARBs) and genes, with the study isolating a multiantibiotic-resistant Acinetobacter lwoffii (N4) from sludge, showing high resistance to various antibiotics and metals.
- - The strain N4 displayed enhanced resistance when exposed to both antibiotics and heavy metals, suggesting a synergistic effect on its resilience against treatments.
- - Whole-genome sequencing revealed 23 antibiotic resistance genes, along with significant virulence factors, indicating N4's potential to be pathogenic and the risks associated with multidrug-resistant bacteria in wastewater systems.
Article Abstract
Pharmaceutical wastewater treatment plants (PWWTPs) serve as reservoirs for antibiotic-resistant bacteria (ARBs) and antibiotic resistance genes (ARGs). In this study, a multiantibiotic-resistant strain of Acinetobacter lwoffii (named N4) was isolated from the dewatered sludge of a PWWTP. N4 exhibited high resistance to both antibiotics and metals, with minimum inhibitory concentrations (MICs) of chloramphenicol and cefazolin reaching 1024 mg·L and MICs of Cu and Zn reaching 512 mg·L. Co-sensitization experiments revealed that when antibiotics are co-existing with heavy metal ions (such as TET and Cd, AMP and Cu) could enhance the resistance of N4 to them. Whole-genome sequencing of N4 revealed a genome size of 0.37 Mb encoding 3359 genes. Among these, 23 ARGs were identified, including dfrA26, bl2be, catB3, qnrB, rosB, tlrC, smeD, smeE, mexE, ceoB, oprN, acrB, adeF, ykkC, ksgA and sul2, which confer resistance through mechanisms such as efflux pumps, enzyme modification and target bypass. Additionally, the N4 genome contained 187 genes associated with human disease and 249 virulence factors, underscoring its potential pathogenicity. Overall, this study provides valuable insights into ARBs in PWWTPs and highlights the potential risks posed by multidrug-resistant strains such as N4.
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| http://dx.doi.org/10.1016/j.ecoenv.2024.117446 | DOI Listing |
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