AI Article Synopsis
- - This study examined how common a specific infection is in layer chickens and looked at the characteristics of the bacteria causing the infection in Sharkia province, Egypt.
- - Out of 160 sampled chickens, 25% tested positive for the infection, with 95% of the bacterial isolates showing extensive drug resistance and significant biofilm production.
- - The research identified strong links between certain genes related to bacterial communication and virulence, suggesting that biofilm production makes bacteria more resistant to antibiotics.
Article Abstract
Background: plays a major influence on poultry outbreaks. Several factors may contribute to its pathogenicity.
Aims: This study aimed to investigate the prevalence of infection among layer chickens with phenotypic and genotypic characterization of the isolates.
Methods: Samples (n=160) were collected from respiratory distressed layer chickens according to the lesion and bacteriologically examined for isolation of from Sharkia province, Egypt. The antimicrobial sensitivity was performed against 18 antimicrobial agents. A qualitative assessment of biofilm production was performed using the Tube method. The isolates were genetically examined for confirmation, detection of quorum sensing genes, virulence genes, and biofilm production genes by conventional PCR.
Results: was isolated from 25% of the samples. Moreover, 95% of the isolates were extensively drug-resistant (XDR) with multiple antibiotic resistance indices (MARI) of 0.67 to 0.83. A total of 38 isolates were able to produce biofilm with different degrees. PCR of () and L genes confirmed the existence of isolates. For quorum sensing genes, and were successfully amplified at 100% and 89.5%, respectively. For virulence genes, A and U were amplified by a percentage of 78.9%, while the gene was in 100% of the isolates. A and B genes were amplified at 100% and 89.5%, respectively. For biofilm genes, A, C, and A were amplified in 100%, 84.2%, and 10.5%, respectively.
Conclusion: A strong correlation between quorum sensing genes, biofilm genes, and virulence genes was detected. Further, biofilm production increases the resistance of the isolates to antimicrobial agents.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11608533 | PMC |
| http://dx.doi.org/10.22099/IJVR.2024.47975.6969 | DOI Listing |
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