Chembiochem
Department of Chemistry, College of Chemistry and Chemical Engineering, Xiamen University, The MOE Key Laboratory of Spectrochemical Analysis and Instrumentation, Xiamen, 361005, China.
Published: December 2024
We report the creation of multivalent ligand surfaces for cell capture by conjugation of ligand-appended 2D peptide assemblies on an antifouling glass substrate. The sheet-like structures organize ligands into non-uniform, patchy patterns, enhancing multivalent cell targeting. A 155 % increase in captured cells was achieved compared to the presentation of the ligands on surfaces lacking the peptide sheets. Being orthogonal to the commonly used dendrimer- and cyclic organic molecular-based scaffolds, this peptide assembly-based approach offers a facile method to modulate the identity, number, and spatial distribution of ligands through controlled peptide coassembly. Using this method, we constructed a surface bearing two types of ligands, which demonstrates a 128 % enhancement in targeting selectivity between two model cells compared to the mono-ligand surface. These findings illustrate that integration of peptide assemblies into ligand substrates permits robust and effective manipulation of multivalent cell targeting, advancing the development of customizable cell-binding materials.
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http://dx.doi.org/10.1002/cbic.202400797 | DOI Listing |
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