The karyotype of (Muesebeck, 1938), an important parasitoid of a serious tomato pest (= ) Meyrick, 1917 (Lepidoptera, Gelechiidae), in the Neotropics and adjacent regions, was studied for the first time using morphometric analysis and several techniques of differential chromosome staining, i.e., C-banding and staining with base-specific fluorochromes, together with fluorescence hybridization (FISH) with an 18S rDNA probe. We found n = 7 and 2n = 14 in , with seven metacentric chromosomes of similar size in the haploid set. C-banding revealed various C-positive bands, either centromeric or interstitial, on most chromosomes. Both AT-specific and GC-specific fluorochromes, 4'6-diamidino-2-phenylindole (DAPI) and chromomycin A (CMA) respectively, showed uniform staining of chromosomes. FISH visualized a single subterminal rDNA site on a medium-sized metacentric. A brief review of known chromosome sets of the subfamily Microgastrinae (Braconidae) is given; certain features of karyotype evolution of this group are discussed.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11605297 | PMC |
http://dx.doi.org/10.3897/compcytogen.18.133534 | DOI Listing |
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