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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11609583 | PMC |
| http://dx.doi.org/10.1002/2211-5463.13943 | DOI Listing |
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Broth microdilution protocol for determining antimicrobial susceptibility of Legionella pneumophila to clinically relevant antimicrobials.
J Microbiol Methods
January 2025
Medical Microbiology, Division of Infection and Immunity, Cardiff University, Cardiff, UK; UK Health Security Agency, Bacteriology Reference Department, London, UK. Electronic address:
Currently there is no detailed, internationally agreed protocol defined to evaluate antimicrobial susceptibility testing (AST) for Legionella pneumophila (required to establish epidemiological cut-off value or "ECOFF" boundaries); therefore, antimicrobial resistance in these isolates cannot be defined. AST methods utilising media containing activated charcoal as an ingredient, to enable Legionella growth, are unreliable as noted in an internationally authored opinion paper and a new gold standard is required. Here we define a detailed protocol for broth microdilution (BMD) using defined cell culture collection-deposited control reference strains (Philadelphia-1 and Knoxville-1) as well as two accessible reference strains with moderately (lpeAB-carrying) and markedly (23S rRNA mutation-carrying) elevated azithromycin minimum inhibitory concentration (MIC).
View Article and Find Full Text PDFIn Reply: Diabetic retinopathy: Screening, prevention, and treatment.
Cleve Clin J Med
December 2024
Vice President, Chief Medical Officer, Cleveland Clinic Martin Hospitals, Cleveland Clinic Florida, Stuart, FL; Professor, Cleveland Clinic Lerner College of Medicine of Case Western Reserve University, Cleveland, OH.
Professor Stuart John Ferguson (29 September 1949-25 April 2024).
FEBS Open Bio
December 2024
Department of Paediatrics, University of Oxford, UK.
High-Voltage Electrical Burn Requiring Urgent Scalp Reconstruction after Developing a Brain Abscess.
Eur Burn J
September 2024
Department of Plastic and Reconstructive Surgery, The Royal Brisbane and Women's Hospital, Brisbane 4029, Australia.
Article Synopsis
- A patient suffered a severe electrical burn to the scalp from a high-voltage power line while cherry picking, resulting in significant tissue damage and an extradural abscess.* -
- The patient's condition included progressive global weakness, and he required urgent surgery to manage the infection and reconstruct the scalp using a free myocutaneous anterolateral thigh flap.* -
- Post-surgery, there were no complications, but the patient experienced limited functional recovery and now needs full-time care, being able to mobilize independently in a wheelchair.*
Online radiology teaching for doctors in a university setting: an Australian experience.
Curr Probl Diagn Radiol
October 2024
School of Health Sciences, University of Sydney, NSW, Australia; Imaging and Phenotyping Laboratory, Charles Perkins Centre, Faculty of Medicine and Health, University of Sydney, NSW, Australia; Lumus Imaging, St George Private Hospital, Kogarah, NSW, Australia. Electronic address:
Background: Imaging Surgical Patients (ISP) and Essential Imaging for Clinicians (EIC) are online medical imaging units of study for non-radiologist medical practitioners within the Master of Surgery and Master of Medicine programs at the University of Sydney. This paper presents the findings of evaluations of the coursework associated with these units.
Methods: ISP and EIC were reviewed after successful delivery of 8 and 4 semesters, respectively.
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