Porcine reproductive and respiratory syndrome virus (PRRSV) is an important pathogen in the swine industry. We report for the first time the detection of NADC34-like PRRSV strains in Japan. Serum samples from 18 piglets in Okinawa Prefecture were analyzed for the open reading frame 5 (ORF5) gene encoding glycoprotein 5, which revealed that all samples belonged to sublineage 1.5 (L1A) of Lineage 1. Phylogenetic analysis, restriction fragment length polymorphism patterns, and glycosylation site characteristics confirmed the presence of NADC34-like strains that caused severe outbreaks in the U.S. and Asia. Despite its known pathogenicity, the farm showed only a transient increase in mortality. These findings highlight the need for further investigation into the pathogenicity of PRRSV strains and their potential for the emergence of new variants in Japan.
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http://dx.doi.org/10.1292/jvms.24-0451 | DOI Listing |
Vet Microbiol
January 2025
College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China; Joint International Research Laboratory of Agriculture and Agri-Product Safety, Yangzhou 225009, China; International Research Laboratory of Prevention and Control of Important Animal Infectious Diseases and Zoonotic Diseases of Jiangsu Higher Education Institutions, Yangzhou 225009, China; Comparative Medicine Research Institute, Yangzhou University, Yangzhou 225009, China; Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou 225009, China. Electronic address:
NADC34-like porcine reproductive and respiratory syndrome virus 2 (NADC34-like PRRSV-2) is currently a major prevalent strain in Chinese swine industry. Within which, recombination events are frequently detected. Previous studies have shown that the pathogenicity of NADC34-like PRRSV-2 isolates is highly variable.
View Article and Find Full Text PDFJ Anim Sci
January 2025
Animal Science Department, University of Nebraska - Lincoln, Lincoln, NE 68583, USA.
Porcine reproductive and respiratory syndrome virus (PRRSV) is the causative agent of a syndrome characterized by reproductive failure and respiratory complications (PRRS). Early detection and classification of PRRSV strains are vital for appropriate management strategies to minimize loss following outbreaks. The most widely used classification method for PRRSV is based on open reading frame 5 (ORF5) sequences.
View Article and Find Full Text PDFFront Vet Sci
December 2024
Changchun Veterinary Research Institute, State Key Laboratory of Pathogen and Biosecurity, Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Chinese Academy of Agricultural Sciences, Changchun, China.
Introduction: Porcine reproductive and respiratory syndrome virus (PRRSV) causes reproductive and respiratory diseases in sow herds and piglets. The emergence of ORF5 RFLP 1-7-4-like (NADC34-like) PRRSV strain in China has brought a new round of challenges to PRRSV prevention.
Methods: In addition, recombinant adenovirus vaccine candidates against the newly emerged NADC34-like strain were constructed in the study; the immunogenicity of the vaccine was investigated in piglets.
Int J Mol Sci
December 2024
Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Porcine reproductive and respiratory syndrome virus (PRRSV) is the pathogen that causes porcine reproductive and respiratory syndrome (PRRS), leading to abortion of sows and the manifestation of respiratory diseases in piglets. PRRSV strains are categorized into two distinct genotypes: PRRSV-1 and PRRSV-2. PRRSV-2 can be further classified into several lineages, including sub-lineage 1.
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