AI Article Synopsis
- The study aimed to evaluate how red blood cells (RBCs) can be coated with penicillin and to analyze the immune reactions in Thai patients' blood samples against these coated RBCs.
- It was found that penicillin-induced immunologic hemolytic anemia (IHA) may be linked to antibodies (IgG) that attack penicillin-coated RBCs, with evidence of some donors also having low levels of penicillin antibodies (often IgM).
- Results showed that out of 304 patients, 17 (5.59%) tested positive for antipenicillin using coated RBCs, while only 3 (1.22%) of 246 blood donors showed positive reactions; importantly, no links were found between patient characteristics
Article Abstract
Objectives: The study aimed to assess the conditions for coating RBCs with penicillin and examine the anti-penicillin reactions of random Thai patients' sera against penicillin-coated RBCs and normal sera from Thai donors testing in the presence of the drug.
Background: Penicillin-induced immunologic haemolytic anaemia (IHA) is reportedly related to possessing antipenicillin antibodies, immunoglobulin G (IgG), which has been identified in testing penicillin-coated red blood cells (RBCs). In addition, low titre penicillin antibodies, often IgM, are detected in donors by testing in the presence of a solution of the penicillin.
Materials And Methods: Penicillin-coated RBCs were produced, and antipenicillin was tested against those penicillin-coated RBCs amongst random Thai patients who had strongly positive direct antiglobulin (≥3+). Additionally, sera from Thai blood donors were tested in the presence of the penicillin. These relationships were determined by comparing the numbers of penicillin-antibody positive patients with their diagnosis, sex, age and blood type.
Results: Penicillin requires a high pH to optimally adhere to RBCs that showed validated reactions with controls. Enrolment of 304 random patients, of whom 17 (5.59%) had positive antipenicillin tests using penicillin-coated RBCs. Of the 246 donor samples, 3 (1.22%) displayed positive reactivities in the presence of soluble penicillin. Furthermore, no association was discovered between the patient's characteristics and antipenicillin positivity.
Conclusions: This is the first study to develop and report on the low percentage of patients' and donors' sera without IHA. Investigating suspected cases of penicillin-induced IHA requires following our suggested method to identify clinically significant antipenicillin.
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| http://dx.doi.org/10.1111/tme.13117 | DOI Listing |
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Article Synopsis
- The study aimed to evaluate how red blood cells (RBCs) can be coated with penicillin and to analyze the immune reactions in Thai patients' blood samples against these coated RBCs.
- It was found that penicillin-induced immunologic hemolytic anemia (IHA) may be linked to antibodies (IgG) that attack penicillin-coated RBCs, with evidence of some donors also having low levels of penicillin antibodies (often IgM).
- Results showed that out of 304 patients, 17 (5.59%) tested positive for antipenicillin using coated RBCs, while only 3 (1.22%) of 246 blood donors showed positive reactions; importantly, no links were found between patient characteristics
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