DcWRKY15 positively regulates anthocyanin biosynthesis during petal coloration in Dianthus caryophyllus.

Plant Physiol Biochem

Frontiers Science Center for Transformative Molecules, Joint International Research Laboratory of Metabolic & Developmental Sciences, Plant Biotechnology Research Center, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, China. Electronic address:

Published: November 2024

Petal pigmentation in carnations is closely associated with the biosynthesis of anthocyanins. This biosynthetic process is tightly regulated by transcription factors, which activate or repress key genes involved in anthocyanin production. Here, we aim to explore the mechanisms involved in the transcriptional regulation of anthocyanin biosynthesis in carnation petals. We identified DcWRKY15 as a critical regulator influencing anthocyanin production in these petals. DcWRKY15 expression showed a strong correlation with the expression levels of genes associated with anthocyanin biosynthesis, peaking during early petal development stages. The findings from the dual-LUC, VIGS, Y1H and EMSA assays demonstrated that DcWRKY15 played a positive regulatory role in anthocyanin biosynthesis. DcWRKY15 achieved this by binding directly to the promoters of DcCHS and DcF3H, thereby enhancing their expression. Additionally, DcWRKY15 interacts with the repressor DcMYB2, which reduces its capacity to enhance anthocyanin biosynthesis, particularly during the later stages of petal development. These findings offer new insights into the molecular mechanisms responsible for petal coloration in carnations, highlighting the complex interplay between activator and repressor transcription factors.

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Source
http://dx.doi.org/10.1016/j.plaphy.2024.109358DOI Listing

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