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Filename: controllers/Detail.php
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Filename: controllers/Detail.php
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File: /var/www/html/application/controllers/Detail.php
Line: 249
Function: _error_handler
File: /var/www/html/index.php
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Severity: Warning
Message: Trying to access array offset on value of type null
Filename: controllers/Detail.php
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File: /var/www/html/application/controllers/Detail.php
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Function: _error_handler
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Filename: controllers/Detail.php
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Filename: models/Detail_model.php
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File: /var/www/html/index.php
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Filename: controllers/Detail.php
Line Number: 260
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Function: require_once
Background/purpose: Nanobacteria, known to date as self-replicating, nano-scale size organisms, smaller than bacteria. However, whether these are living organisms or agglomeration of biomolecules was one of the most controversial issues for many years. One of the reasons for debate is their lack of any genetic material. Although many studies investigate whether nanobacteria are the cause of diseases that affect human health, the discussions did not reach a conclusion. While most studies try to unravel the structures of nanobacteria, some studies investigated their effects on diseases. It has been proposed that pathological calcifications in kidney and dental pulp stones, as well as calcifications in heart valves and arteries, might be caused by nanobacteria. In this study, we investigated the effects and molecular structures of nanobacteria on the formation of atheroma plaques that cause atherosclerosis.
Methods: Nanobacteria grown from atheroma plaques in cell culture medium were analysed by scanning electron microscope (SEM) and energy dispersive X-ray spectroscopy (EDS). Macromolecules of nanobacteria were separated by SDS-polyacrylamide gel electrophoresis and their sensitivity to protease and nucleases was tested. After determining their resistance to nucleases and susceptibility to proteinase K, protein profiles were determined by quadrupole time-of-flight mass spectrometry (QTOF-MS).
Results: In SEM, spherical structures with sizes ranging from 80 to 900 nm were visualized. EDS analyses showed that very low concentration of phosphorus (P: 0.3 %) indicated absence of nucleic acids. The resistance was observed to proteases. Also, protein profiles indicated human proteins, with a high percentage of albumin.
Conclusion: It was determined that nanobacteria derived from atheroma plaque were structures composed of human proteins. The findings confirmed the theory that nanobacteria are non-living structures formed by a mechanism called biocrystallization. Although the accumulation of nanobacteria may be a contributing element to the creation of atheroma plaque, it is possible that the etiological components that initiate the formation of the plaque are not nanobacteria.
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http://dx.doi.org/10.1016/j.ecoenv.2024.117415 | DOI Listing |
Ecotoxicol Environ Saf
December 2024
Acibadem Mehmet Ali Aydınlar University, Institute of Health Sciences, Department of Medical Biotechnology, Istanbul, Turkey; Acibadem Mehmet Ali Aydınlar University, School of Medicine, Department of Medical Microbiology, Istanbul, Turkey. Electronic address:
Background/purpose: Nanobacteria, known to date as self-replicating, nano-scale size organisms, smaller than bacteria. However, whether these are living organisms or agglomeration of biomolecules was one of the most controversial issues for many years. One of the reasons for debate is their lack of any genetic material.
View Article and Find Full Text PDFACS Appl Mater Interfaces
November 2024
Biomedical Polymers Laboratory, College of Chemistry, Chemical Engineering and Materials Science, and State Key Laboratory of Radiation Medicine and Protection, Soochow University, Suzhou 215123, P.R. China.
The clinical efficacy of cancer vaccines is closely related to immunoadjuvants that play a crucial role in magnifying and prolonging the immune response. Muramyl dipeptide (MDP), a minimal and conserved peptidoglycan found in almost all bacteria, can trigger robust immune activation by uniquely antagonizing the nucleotide-binding oligomerization domain 2 (NOD2) pathway. However, its effectiveness has been hindered by limited solubility, poor membrane penetration, and rapid clearance from the body.
View Article and Find Full Text PDFJ Infect Public Health
October 2024
Acibadem University, Institute of Health Sciences, Department of Medical Biotechnology, Istanbul, Turkey; Acibadem University, School of Medicine, Department of Medical Microbiology, Istanbul, Turkey. Electronic address:
Background: Cardiovascular diseases (CVDs) are the leading cause of death worldwide. Vital organs like the heart are affected by the occlusion of blood vessels due to atherosclerotic plaque formation. However, the role of infectious agents has always been an essential subject of investigation.
View Article and Find Full Text PDFCardiovasc Res
December 2024
Department of Pathology and Medical Biology, University of Groningen, University Medical Center Groningen, Hanzeplein 1, 9713 GZ Groningen, The Netherlands.
Aims: Calciprotein particles (CPPs) are circulating calcium and phosphate nanoparticles associated with the development of vascular calcification (VC) in chronic kidney disease (CKD). Although recent studies have been focusing on associations of CPPs with the presence of VC in CKD, insights in the underlying processes and mechanisms by which CPPs might aggravate VC and vascular dysfunction in vivo are currently lacking. Here, we assessed the overall burden of abdominal VC in healthy kidney donors and CKD patients and subsequently performed transcriptome profiling in the vascular tissue obtained from these subjects, linking outcome to CPP counts and calcification propensity.
View Article and Find Full Text PDFClin Exp Dent Res
June 2024
Key Laboratory of Oral Disease of Higher Schools in Guizhou Province, Zunyi Medical University, Zunyi, China.
Objectives: Calcifying nanoparticles (CNPs), referred to as nanobacteria (NB), are recognized to be associated with ectopic calcification. This study aims to isolate and culture CNPs from the dental plaque of patients with periodontal disease and investigate their possible role in unravelling the aetiology of periodontal disease.
Material And Methods: Supragingival and subgingival plaques were sampled from 30 periodontitis patients for CNPs isolation and culture.
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