Submergence tolerance is a complex trait governed by multiple genetic loci. Arabidopsis thaliana, widely distributed from arid to humid regions, offers an opportunity to explore the underlying genetic components and their interactive mechanisms. In this study, we utilized map-based cloning techniques to identify the WRKY22 genetic locus, which activates RAP2.12, a locus identified previously, possibly through ethylene signal transduction. WRKY22 expression is inhibited by ARRs but activated by the WRKY70 transcription factors. The cooperative interaction between these regulators enhances A. thaliana's ability to adapt more effectively to contrasting habitats. A two-nucleotide deletion in the WRKY22 promoter region prevents its inhibition by phosphorylated ARRs, maintaining its high expression in humid accessions. This WRKY22 allele without ARRs binding element underwent strong natural selection during the southeastern colonization of A. thaliana to the humid Yangtze River basin. However, the WRKY70-activated WT box in the WRKY22 promoter region shows no variation between humid and arid accessions. These findings together establish one previously unreported molecular module composed by multiple regulators, with WRKY22 playing a key role in the ecological shift of A. thaliana. Expanding upon the identified locus, we overexpressed BnaC2.WRKY22 in Brassica napus and successfully achieved a significant improvement in submergence tolerance, highlighting a functional conservatism of this gene across related species. This further demonstrates the potential for transferring knowledge from model plants to crops for the breeding of flood-tolerant crop varieties.
Download full-text PDF |
Source |
---|---|
http://dx.doi.org/10.1016/j.molp.2024.11.013 | DOI Listing |
BMC Plant Biol
December 2024
College of Life Sciences, Engineering Research Center of the Chinese Ministry of Education for Bioreactor and Pharmaceutical Development, Changchun, 130118, China.
Background: Safflower thrives in dry environments but faces difficulties with flowering in wet and rainy summers. Flavonoids play a role in flower development and can potentially alleviate these challenges. Furthermore, the FLOWERING LOCUS T (FT) family of phosphatidylethanolamine-binding protein (PEBP) genes play a crucial role in the photoperiodic flowering pathway.
View Article and Find Full Text PDFPhysiol Plant
December 2024
Department of Animal Biology, Plant Biology and Ecology; Plant Physiology Lab, Universitat Autònoma de Barcelona, Barcelona, Spain.
Carbonic anhydrases (CAs) are the main enzymes handling bicarbonate in the different cell compartments. This study analyses the expression of CAs in roots of Arabidopsis thaliana demes differing in tolerance to bicarbonate: the tolerant A1 deme and the sensitive deme, T6. Exposure to 10 mM NaCl caused a transient depolarization of the root cell membranes, and in contrast, the supply of 10 mM NaHCO caused hyperpolarization.
View Article and Find Full Text PDFPlant Sci
December 2024
State Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, Henan, China; Zhengzhou Research Base, State Key Laboratory of Cotton Bio-breeding and Integrated Utilization, School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450001, Henan, China. Electronic address:
Cotton is an important source of natural fibers. The AP2/ethylene response factor (ERF) family is one of the largest plant-specific transcription factors (TFs) groups, playing key roles in plant growth and development. However, the role of ERF TFs in cotton's growth and development remains unclear.
View Article and Find Full Text PDFPlant Sci
December 2024
School of Life Sciences, Shaanxi Normal University, Xi'an 710119, China. Electronic address:
In animal cells, Gα subunit of the heterotrimeric G proteins can bind to both the N-terminal and C-terminal domains of G-protein-activated inwardly rectifying K channels (GIRKs) to inhibit their activities. In Arabidopsis guard cells, the Gα subunit GPA1 mediates multiple stimuli-regulated stomatal movements via inhibiting guard cell inward-rectifying K (K) current, but it remains unclear whether GPA1 directly interacts with and inhibits the activities of K channels. Here, we found that GPA1 interacted with the transmembrane domain rather than the intracellular domain of the Shaker family K channel KAT1.
View Article and Find Full Text PDFPlant Physiol
December 2024
Departamento de Genética Molecular de Plantas, Centro Nacional de Biotecnología (CNB), Consejo Superior de Investigaciones Científicas (CSIC), Madrid 28049, Spain.
The number and distribution of trichomes, i.e., the trichome pattern, in different plant organs shows a conspicuous inter- and intraspecific diversity across Angiosperms that is presumably involved in adaptation to numerous environmental factors.
View Article and Find Full Text PDFEnter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!