AI Article Synopsis
- Time-lapse microscopy is an essential method for studying cell cycle dynamics, particularly to observe the duration of anaphase in budding yeast cells.
- The chapter provides a detailed guide on using this technique, covering everything from preparing samples and setting up the microscope to analyzing the data.
- By focusing on the mitotic spindle, the methodology helps researchers understand the kinetics of mitotic exit in cell division.
Article Abstract
Time-lapse microscopy is a valuable, widely employed technique for investigating cell cycle dynamics. This chapter will guide you in using this tool to determine the duration of anaphase as a measure of mitotic exit kinetics in budding yeast cells. The methodology explained here is based on monitoring the mitotic spindle and provides a comprehensive guide covering all aspects, from sample preparation and microscopy to data analysis.
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| http://dx.doi.org/10.1007/978-1-0716-4236-8_6 | DOI Listing |
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