AI Article Synopsis
- Cryopreservation of neural organoids and human brain tissue is complex, but a new method using a solution called MEDY has been developed.
- The MEDY protocol includes preparing the cryopreservation solution, creating cortical organoids, and surgically extracting human brain tissue.
- The protocol also outlines steps for cryopreservation, resuscitation, and detection assays, making it a valuable tool for researching human neurological diseases.
Article Abstract
Cryopreservation of neural organoids and human living brain tissue faces huge challenges. Here, we present a cryopreservation protocol for the reliable storage of diverse neural organoids and human living brain tissue using methylcellulose, ethylene glycol, DMSO, and Y27632, termed MEDY. We describe steps for preparing cryopreservation solution, preparing cortical organoids, and surgical removal of human brain tissue. We then detail procedures for cryopreservation, resuscitation, and detection assays. This protocol can serve as a model for studying human neurological diseases. For complete details on the use and execution of this protocol, please refer to Xue et al..
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11636082 | PMC |
| http://dx.doi.org/10.1016/j.xpro.2024.103466 | DOI Listing |
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