AI Article Synopsis
- Investigates how microtubule growth and stability are affected by a stabilizing GTP/GDP-Pi cap using in vitro experiments with microfabricated barriers and GFP-EB3 as a marker.
- The study reveals that obstacles slow down microtubule growth and increase the likelihood of catastrophes (rapid depolymerization).
- A simple 1D model can effectively describe the lifetimes of both stalled and freely growing microtubules, linking hydrolysis rates to their growth dynamics and explaining previous observations on catastrophe rates.
Article Abstract
Microtubule stability is known to be governed by a stabilizing GTP/GDP-Pi cap, but the exact relation between growth velocity, GTP hydrolysis, and catastrophes remains unclear. We investigate the dynamics of the stabilizing cap through in vitro reconstitution of microtubule dynamics in contact with microfabricated barriers, using the plus-end binding protein GFP-EB3 as a marker for the nucleotide state of the tip. The interaction of growing microtubules with steric objects is known to slow down microtubule growth and accelerate catastrophes. We show that the lifetime distributions of stalled microtubules, as well as the corresponding lifetime distributions of freely growing microtubules, can be fully described with a simple phenomenological 1D model based on noisy microtubule growth and a single EB3-dependent hydrolysis rate. This same model is furthermore capable of explaining both the previously reported mild catastrophe dependence on microtubule growth rates and the catastrophe statistics during tubulin washout experiments.
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| http://dx.doi.org/10.1016/j.bpj.2024.11.3314 | DOI Listing |
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