is an opportunistic human fungal pathogen that causes superficial mucosal and life-threatening bloodstream infections in immunocompromised individuals. Remodeling in cell wall components has been extensively exploited by fungal pathogens to adapt to host-derived stresses, as well as immune evasion. How this process contributes to pathogenicity is less understood. Here, we applied RNA sequencing and an invasive infection model to elucidate the prompt response of during infection. Fungal transcriptomes show a dramatic alteration in the expression of Srp1/Tip1-family cell wall structural proteins during systemic infection. Deletion of all six genes in this family ( and ) that are upregulated during infection leads to a significantly lower fungal burden in organs, as well as an attenuated virulence in the dextran sulfate sodium-induced colitis model. The sextuple mutant does not display any defect in response to host-derived stresses. Rather, deletion of all these six genes results in a lower cell surface exposure of an adhesin Epa1, which could contribute to its reduced adhesion to epithelial cells and cytotoxicity, as well as attenuated virulence. Our study reveals that cell wall remodeling triggered by the alteration in the expression of structural proteins is a key virulence attribute in that facilitates this fungus adhering to host cells and persisting in organs.IMPORTANCE is one of the most frequent causes of candidiasis after . While has been extensively studied, the mechanisms of infection and invasion of have not been fully elucidated. Using an infection model of systemic candidiasis and RNA sequencing, we show that there is a dramatic change in the expression of Srp1/Tip1-family genes during infection. Deletion of all six Srp1/Tip1-family genes that are upregulated during infection decreases the amount of cell wall-localized Epa1, probably reflecting the reduced adherence to epithelial cells and attenuated virulence in the sextuple mutant. These data suggest that alterations in the expression of Srp1/Tip1-family structural proteins trigger cell wall remodeling that increases the cell surface exposure of adhesins, such as Epa1, to promote virulence. Our study provides a pathogenic mechanism associated with in ensuring its sustenance and survival during infection.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11656777 | PMC |
Microbiome
December 2024
MOE Key Laboratory of Bio-Intelligent Manufacturing, School of Bioengineering, Dalian University of Technology, Dalian, Liaoning, 116024, China.
Background: The overuse of antibiotics has led to lethal multi-antibiotic-resistant microorganisms around the globe, with restricted availability of novel antibiotics. Compared to conventional antibiotics, evolutionarily originated antimicrobial peptides (AMPs) are promising alternatives to address these issues. The gut microbiome of Blattella germanica represents a previously untapped resource of naturally evolving AMPs for developing antimicrobial agents.
View Article and Find Full Text PDFJ Genet Genomics
December 2024
Zhejiang Provincial Key Laboratory of Crop Genetic Resources, Key Lab of Plant factory for Plant Factory Generation-Adding Breeding of Ministry of Agriculture and Rural Affairs, the Advanced Seed Institute, Zhejiang University, Hangzhou, Zhejiang 310058, China; Hainan Institute of Zhejiang University, Sanya, Hainan 572025, China. Electronic address:
The Malvaceae family, the most diverse family in the order Malvales, consists of nine subfamilies. Within the Firmiana genus of the Sterculioideae subfamily, most species are considered globally vulnerable, yet their genomes remain unexplored. Here, we present a chromosome-level genome assembly for a representative Firmiana species, F.
View Article and Find Full Text PDFInt J Biol Macromol
December 2024
College of Horticulture, South China Agricultural University, Guangzhou 510642, China. Electronic address:
Fasciclin-like arabinogalactan proteins (FLAs) have been shown to improve plant tolerance to salt stress. However, their role in cold tolerance (CT) remains unclear. Here, we report that banana MaFLA27 positively regulates CT in Arabidopsis.
View Article and Find Full Text PDFJ Colloid Interface Sci
December 2024
Liaoning Province Key Laboratory of Pulp and Papermaking Engineering, Liaoning Key Lab of Lignocellulose Chemistry and BioMaterials, Liaoning Collaborative Innovation Center for Lignocellulosic Biorefinery, Dalian Polytechnic University, Dalian, Liaoning Province, 116034, China; Shandong Tonye Photoresist Material Technology CO., LTD, Weifang, 261206, China. Electronic address:
The poor mechanical properties of biomass-based carbon aerogels after carbonization severely limit their application in pressure sensing and energy storage for wearable devices and electronic skin. In this work, a supramolecular assembly structure was designed inspired by the unique microstructure of natural wood for the preparation of biomass-based carbon aerogels with supercompressibility, elasticity, stable strain electrical signal response, and wide sensitive detection. Bacterial cellulose and lignin were selected as the main components of the biomass-based composite aerogel 'cell wall'.
View Article and Find Full Text PDFEcotoxicol Environ Saf
December 2024
Henan Agricultural University, Zhengzhou, Henan 450002, China. Electronic address:
Copper (Cu) pollution resulting from human activities has emerged as a prominent issue stemming from industrial development. The entry of copper into the soil, either directly or through industrial wastewater, exerts adverse effects on plant growth, leading to its accumulation in significant quantities within the plant and subsequent endangerment of human health through the food chain. DCPTA [2-(3, 4-dichlorophenoxy) triethylamine] mitigates certain abiotic stresses, yet the precise mechanism by which it alleviates Cu-induced phytotoxicity remains unknown.
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