Background And Objectives: Expansion of white adipose tissue causes systemic inflammation and increased risk of metabolic diseases due to its endocrine function. Resveratrol was suggested to be able to prevent obesity-related disorders by mimicking caloric restriction; however, its structure-activity relationships and molecular targets are still unknown. We aimed to compare the effects of resveratrol and its analogues on adipocyte metabolism and lipid accumulation in vitro.
Methods: Mouse embryonic fibroblasts were differentiated to adipocytes in the absence or presence of resveratrol or its derivatives (oxyresveratrol, monomethylated resveratrol, or trimethylated resveratrol). Intracellular lipid content was assessed by Oil Red O staining. Glucose uptake and its response to insulin were estimated by 2-NBDG, and mitochondrial activity was assayed via resazurin reduction. Involvement of potential molecular pathways was investigated by concurrent treatment with their inhibitors.
Results: Although lipid accumulation was significantly reduced by all analogues without altering protein content, oxyresveratrol was the most potent (IC50 = 4.2 μM), while the lowest potency was observed with trimethylated resveratrol (IC50 = 27.4 μM). Increased insulin-stimulated glucose uptake was restored by each analogue with comparable efficiency. The enhanced mitochondrial activity was normalized by resveratrol and its methylated derivatives, while oxyresveratrol had a minor impact on it. Among the examined pathways, inhibition of SIRT1, PGC-1α, and JNK diminished the lipid-reducing effect of the compounds. Autophagy appeared to play a key role in the effect of all compounds but oxyresveratrol.
Conclusions: Resveratrol and its analogues can mimic caloric restriction with complex mechanisms, including activation of SIRT1, PGC-1α, and JNK, making them possible drug candidates to treat obesity-related diseases.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11597095 | PMC |
| http://dx.doi.org/10.3390/nu16223869 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Pro-renin Receptor (PRR) as a Target to Reduce Non-alcoholic Fatty Liver Disease Post Liver Transplantation.
Curr Med Chem
January 2025
Transplant Research Center, Clinical Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran.
Nonalcoholic fatty liver disease (NAFLD) is one of the main causes of chronic liver disorders following liver transplantation. The prorenin receptor (PRR) plays a role in glucose and lipid metabolism, and the hepatic dysregulation of PRR is associated with the upregulation of several molecular pathways, such as the mammalian target of rapamycin (mTOR) and Peroxisome proliferator-activated receptor (PPAR) that promotes hepatic lipogenesis and leads to lipid accumulation in hepatocytes by upregulation of lipogenic genes. PRR inhibition leads to a reduction in the hepatic expression of sortilin-1 and low-density lipoprotein receptor (LDLR) levels and down-regulation of pyruvate dehydrogenase (PDH) and acetyl-CoA carboxylase (ACC) and reduces fatty acids synthesis in hepatocytes.
View Article and Find Full Text PDFMelatonin improves endometrial receptivity and embryo implantation via MT2/PI3K/LIF signaling pathway in sows.
J Anim Sci Biotechnol
January 2025
Key Laboratory of Northwest China's Pig Breading and Reproduction, Ministry of Agriculture and Rural Affairs of the People's Republic of China, College of Animal Science and Technology, Northwest A&F University, Yangling, 712100, Shaanxi, China.
Background: Increased backfat thickness of sows in early gestation is negative to reproductive performance. Endometrial receptivity is an important determinant of reproductive success, but it is unclear whether the effect of sow backfat thickness on litter size is associated with endometrial receptivity and whether melatonin treatment may have benefits. The present study seeks to answer these questions through in vitro and in vivo investigations.
View Article and Find Full Text PDFMTCH2 controls energy demand and expenditure to fuel anabolism during adipogenesis.
EMBO J
January 2025
Department of Immunology and Regenerative Biology, Weizmann Institute of Science, 76100, Rehovot, Israel.
Mitochondrial carrier homolog 2 (MTCH2) is a regulator of apoptosis, mitochondrial dynamics, and metabolism. Loss of MTCH2 results in mitochondrial fragmentation, an increase in whole-body energy utilization, and protection against diet-induced obesity. In this study, we used temporal metabolomics on HeLa cells to show that MTCH2 deletion results in a high ATP demand, an oxidized cellular environment, and elevated utilization of lipids, amino acids, and carbohydrates, accompanied by a decrease in several metabolites.
View Article and Find Full Text PDFConstruction and transcriptomic analysis of salinity-induced lipid-rich flocculent microalgae.
J Environ Manage
January 2025
School of Petrochemical Engineering & Environment, Zhejiang Ocean University, Zhoushan, 316000, China; National & Local Joint Engineering Research Center of Harbor Oil & Gas Storage and Transportation Technology, Zhejiang Key Laboratory of Petrochemical Environmental Pollution Control, Zhoushan, 316000, China. Electronic address:
The lack of cost-effective nutrient sources and harvesting methods is currently a major obstacle to the production of sustainable biofuels from microalgae. In this study, Chlorella pyrenoidosa was cultured with saline wastewater in a stirred photobioreactor, and lipid-rich flocculent microalgae particles were successfully constructed. As the influent salinity of the photobioreactor increased from 0% to 3%, the particle size and sedimentation rate of flocculent microalgae particles gradually increased, and the lipid accumulation of microalgae also increased gradually.
View Article and Find Full Text PDFThe function of SnRK1 in regulating darkness-induced leaf senescence in cucumber.
Plant Physiol Biochem
December 2024
College of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang, 110866, China; Key Laboratory of Fruit and Vegetable Biology and Germplasm Enhancement, Shenyang Agricultural University, Shenyang, 110866, China; Key Laboratory of Protected Horticulture of Ministry of Education, Shenyang Agricultural University, Shenyang, 110866, China. Electronic address:
SnRK1 (SNF1-related kinase 1), a member of the SNF1 protein kinase superfamily, has been demonstrated to play a role in plant growth and development, as well as in stress responses. In this experiment, the leaf senescence of 'Xintaimici' cucumber was simulated by dark treatment and studied using SnRK1 activator/inhibitor and transient transformation technology. The effects of SnRK1 on cucumber leaf senescence, reactive oxygen species (ROS) metabolism, chloroplast structure, and photosynthetic characteristics were studied.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!