AI Article Synopsis
Article Abstract
Background: is a pathogen that causes infections in animals and humans, with veterinary implications including ear infections in dogs, respiratory diseases in cats, and mastitis in ruminants. In humans, it causes severe hospital-acquired infections, particularly in immunosuppressed patients. This study aimed to identify and assess the prevalence of specific virulence factors in isolates.
Methods: We analyzed 98 isolates from various animal samples (dogs, cats, ruminants, fowl) from northeastern Poland in 2019-2022 for virulence-related genes (toxA, exoU, exoT, exoS, lasB, plcN, plcH, pldA, aprA, gacA, algD, pelA, endA, and oprF) by PCR and assessed biofilm formation at 48 and 72 h. Genomic diversity was assessed by ERIC-PCR.
Results: The obtained results showed that all strains harbored the A gene (100%), while the lowest prevalence was found for A (24%) and U (36%). Regardless of the animal species, strong biofilm forming ability was prevalent among the strains after both 48 h (75%) and 72 h (74%). We obtained as many as 87 different genotyping profiles, where the dominant one was profile ERIC-48, observed in four strains.
Conclusions: No correlation was found between presence or absence of determined genes and the nature of infection. Similarly, no correlation was found between biofilm-forming genes and biofilm strength. The high genetic diversity indicates challenges for effective prevention, emphasizing the need for ongoing monitoring and research.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11597259 | PMC |
| http://dx.doi.org/10.3390/pathogens13110979 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Overexpression of gene increases the rice resistance to pv. through negatively regulating transcription activator-like effectors translocation.
Plant Signal Behav
December 2025
Institute of Integrative Plant Biology, School of Life Sciences, Jiangsu Normal University, Xuzhou, China.
Bacterial leaf blight (BLB) caused by pv. () has shown a high incidence rate in rice fields in recent years. Rice resistance breeding is considered as the most effective method for achieving economical and sustainable management of BLB disease.
View Article and Find Full Text PDFUnraveling the genomic landscape of piscine myocarditis virus: mutation frequencies, viral diversity and evolutionary dynamics in Atlantic salmon.
Virus Evol
November 2024
Department of Paraclinical Sciences, Norwegian University of Life Sciences, Post box 5003, Ås 1432, Norway.
Over a decade since its discovery, piscine myocarditis virus (PMCV) remains a significant pathogen in Atlantic salmon aquaculture. Despite this significant impact, the genomic landscape, evolutionary dynamics, and virulence factors of PMCV are poorly understood. This study enhances the existing PMCV sequence dataset by adding 34 genome sequences and 202 new ORF3 sequences from clinical cardiomyopathy syndrome (CMS) cases in Norwegian aquaculture.
View Article and Find Full Text PDFImmunoinformatics-driven design and computational analysis of a multiepitope vaccine targeting uropathogenic .
In Silico Pharmacol
December 2024
Laboratory of Chemoinformatics, Infochemistry Scientific Center, ITMO University, Saint Petersburg, Russian Federation.
Unlabelled: Urinary tract infections (UTIs), largely caused by uropathogenic (UPEC), are increasingly resistant to antibiotics and frequently recur. Using immunoinformatics, we designed a multiepitope peptide vaccine targeting UPEC virulence factors, including iron acquisition systems and adhesins. The construct features 12 cytotoxic T lymphocyte epitopes, six helper T lymphocyte epitopes, and six B-cell epitopes,and isoptimized for high antigenicity, immunogenicity, nontoxic, and low allergenic potential.
View Article and Find Full Text PDFGenomic analysis, culturing optimization, and characterization of bacteriophage OSYSP, previously studied as effective pathogen control on fresh produce.
Front Microbiol
December 2024
Department of Food Science and Technology, The Ohio State University, Columbus, OH, United States.
Advances in bacteriophage genome sequencing and regulatory approvals of some bacteriophages in various applications have renewed interest in these antibacterial viruses as a potential solution to persistent food safety challenges. Here, we analyzed in depth the genome of the previously studied bacteriophage OSYSP (phage OSYSP), revealed its application-related characteristics, and optimized its enumeration techniques for facilitating industrial implementation. We previously sequenced phage OSYSP genome completely by combining results from Illumina Miseq and Ion Torrent sequencing platforms and completing the remaining sequence gaps using PCR.
View Article and Find Full Text PDFHigh activity and specificity of bacteriophage cocktails against carbapenem-resistant belonging to the high-risk clones CG258 and ST307.
Front Microbiol
December 2024
Grupo de Investigación en Microbiología Básica y Aplicada (MICROBA), Escuela de Microbiología, Universidad de Antioquia, Medellín, Colombia.
Introduction: The widespread clinical and environmental dissemination of successful clones of carbapenem-resistant (CRKP) represents a serious global public health threat. In this context, lytic bacteriophages have emerged as a promising alternative for controlling these pathogens. This study describes the biological, structural, and genomic characteristics of lytic bacteriophages against the high-risk CRKP clones CG258 and ST307 and describes their performance in combination.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!