Overexpression of the Kiwifruit Transcription Factor Decreases the Cold Tolerance in .

Cold stress is one of the main abiotic stresses that affect the development and growth of kiwifruit (). Herein, we analyzed the transcriptomic data of dormant shoots in response to low-temperature treatment, identified 52 MYB genes, and constructed a phylogenetic tree based on the encoded protein sequences. Then, the effect of one gene on cold tolerance was analyzed. This gene had an open reading frame of 837 bp long and encoded 279 amino acids. Sequence alignment and phylogenetic analysis revealed that this gene belongs to the family and was named based on its homology to other family members. Quantitative real-time PCR revealed that expression was significantly induced by low temperatures but exhibited the opposite trend in cold-tolerant genotypes. Subcellular localization assays revealed the nuclear localization of the AaMYB44 protein. Furthermore, was transformed into () via inflorescence infection, and physiological and biochemical tests revealed that the cold resistance and antioxidant capacity of the transgenic were lower than those of wild-type plants. Overall, might play a negative regulatory role in response to cold stress, providing new insight into the mechanism of cold tolerance.