Probiotic and Rice-Derived Compound Combination Mitigates Colitis Severity.

Background: This study investigated the ability of () and Hasawi rice protein lysate (HPL) to suppress colitis induced by dextran sulfate sodium (DSS) in miceColitis is characterized by inflammation of the colon, and exploring potential therapeutic agents could lead to improved management strategies.

Methods: Male mice were subjected to DSS treatment to induce colitis, followed by supplementation with and/or HPL. The study assessed various parameters, including disease activity index (DAI) scores, gut permeability measured using FITC-dextran, and superoxide dismutase (SOD) activity in excised colon tissues from both treated and untreated control groups.

Results: supplementation significantly alleviated DSS-induced colitis, as evidenced by improved DAI scores and enhanced gut permeability. Notably, combined with HPL (0.1 mg/10) exhibited superior tolerance to a 0.5% pancreatin solution compared to alone. Both and the combination treatment significantly increased SOD activity (5.6 ± 0.23 SOD U/mg protein for and 6.7 ± 0.23 SOD U/mg protein for the combination) relative to the Azoxymethane (AOM)/DSS group, suggesting a reduction in oxidative stress. Additionally, pro-inflammatory markers were significantly reduced in the group receiving both and HPL compared to the E. lactis-only group. Levels of proteins associated with cell death, such as PCNA, PTEN, VEGF, COX-2, and STAT-3, were significantly decreased by 14.8% to 80% following supplementation, with the combination treatment showing the most pronounced effects.

Conclusions: These findings suggest supplementation may be beneficial for colitis, with HPL potential to enhance its effectiveness.