Prevalence, Antimicrobial Susceptibility, and Resistance Genes of Extended-Spectrum β-Lactamase-Producing from Broilers Sold in Open Markets of Dakar, Senegal.

Extended-spectrum β-lactamase-producing (ESBL-) poses a significant public health concern due to its widespread prevalence and resistance to multiple antibiotics. This study aimed to assess the prevalence, resistance profile, and carriage of ESBL-encoding genes in ESBL- isolates from broilers in two markets of Dakar, Senegal. Sampling over a one-year period revealed that 61.7% of broilers carried ESBL- in their cecum. Antibiotic susceptibility testing of 186 ESBL- isolates showed high resistance to β-lactam antibiotics, including ampicillin, ticarcillin, and third-generation cephalosporins. Notably, all isolates were susceptible to imipenem. Multidrug resistance was frequent, with 91.4% of the isolates displaying this phenotype. Comparison between the two markets revealed variations in resistance to quinolones. PCR analysis detected genes in all isolates, with being the most prevalent subgroup. Additionally, and genes were found in 26.3% and 2.7% of isolates, respectively, while no genes were detected. Combinations of ESBL genes were common, with being the most frequent. These findings highlight the widespread prevalence of ESBL- in broilers from Dakar markets, indicating a potential risk of transmission to humans through the food chain. Effective surveillance and intervention strategies are crucial to mitigate the spread of antimicrobial resistance in humans and animals.