Over Expression of Mango in Tobacco Reduced Plant Height by Reducing GA and GA Content.

The regulation of gibberellic acid 2-oxidase () gene expression represents a critical mechanism in the modulation of endogenous gibberellic acids (GAs) levels, thereby exerting an influence on plant height. In this context, we conducted a comprehensive genome-wide analysis of the GA2ox gene family in mango ( L.), a species of significant economic importance, with the aim of identifying potential candidate genes for mango dwarf breeding. Our findings delineated the presence of at least 14 members within the gene family in the mango genome, which were further categorized into three subfamilies: C-GA2ox-I, C-GA2ox-II, and C-GA2ox-I. Notably, a member of the C-GA2ox-II subfamily, exhibited substantial expression across various tissues, including roots, bark, leaves, and flowers. Through overexpression of the gene in tobacco, a distinct dwarf phenotype was observed alongside reduced levels of GA and GA, while the knockout line exhibited contrasting traits. This provides evidence suggesting that may exert control over plant height by modulating GA content. Consequently, the gene emerges as a promising candidate for facilitating advancements in mango dwarfing techniques.