AI Article Synopsis
- - This study focuses on creating an optimized method for cryopreserving the germ cells of an endangered snail species using vitrification, which is a new technique for preserving these organisms.
- - Key reproductive organs like the ovotestis, which contains both male and female germ cells, were analyzed, revealing structures such as sperm and early germ cells through specific tissue staining methods.
- - The results indicated that while cryopreservation using vitrification reduced cell viability (86.8%) compared to non-vitrification methods (96.6%), it provides a viable protocol for conserving the genetic resources of endangered snail species, potentially aiding broader biodiversity conservation efforts.
Article Abstract
This study aims to develop an optimized method for cryopreserving the germ cells of () using vitrification, as an alternative approach for conserving endangered snail species. First, we isolated several key reproductive organs, including the sperm oviduct, albumen gland, hermaphrodite gland (ovotestis), and hermaphrodite duct from . When the ovotestis was finely chopped, numerous sperm with long tails and distinct heads were observed. The staining of sperm nuclei was confirmed using Hoechst 33342 dye. Since the hermaphrodite gland, referred to as the ovotestis, contains both male and female germ cells, we performed tissue staining on the ovotestis using hematoxylin and eosin (H&E) dye. H&E staining of the ovotestis revealed numerous oval-shaped acini containing sperm and early germ cells. Spermatocytes and spermatids were observed within distinct boundaries, with mature sperm appearing following spermatogenesis. To preserve the species of the , we introduced vitrification technology to cryopreserve its reproductive organs. The non-vitrification group showed an average cell viability of 96.6%, while the vitrification group had 86.8% after thawing. This study presents a reliable cryopreservation protocol for , with potential applications for other endangered snails, supporting conservation efforts to preserve genetic resources and biodiversity.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11591466 | PMC |
| http://dx.doi.org/10.3390/ani14223229 | DOI Listing |
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