Anti-Melanogenic Activities of Polyphenol-Rich Extract on α-MSH-Stimulated B16F10 Cells via PI3K/Akt and MAPK/ERK Pathways.

Background: Melanin overproduction leads to pigmented skin diseases. Brown algae polyphenols, non-toxic secondary metabolites, exhibit potential bioactivities. , an edible seaweed, has been underexplored in the field of beauty despite its polyphenol richness.

Methods: Polyphenols from were extracted using macroporous resin (SFRP) and ethyl acetate (SFEP). Their antioxidant and anti-aging properties, tyrosinase inhibitory activities, and mechanisms were assessed. The melanogenesis inhibition effect and mechanism by SFRP was examined in B16F10 melanoma cells.

Results: Both SFRP and SFEP demonstrated scavenging activities against DPPH, superoxide anion, and hydroxyl radicals. SFRP showed stronger anti-collagenase and anti-elastase effects. They dose-dependently inhibited mushroom tyrosinase, with IC50 values of 9.89 μg/mL for SFRP and 0.99 μg/mL for SFEP. SFRP reversibly inhibited tyrosinase, while SFEP showed irreversible inhibition. SFRP also suppressed melanin content and intracellular tyrosinase activity in B16F10 cells, downregulating the expression of microphthalmia-associated transcription factor, tyrosinase, and tyrosinase-related protein 1 and 2 expression through the PI3K/Akt and MAPK/ERK signal pathways.

Conclusions: polyphenols, especially SFRP, exhibit promising antioxidant, anti-aging, and melanogenesis inhibitory properties, highlighting their potential application as novel anti-melanogenic agents in cosmetics and the food industry.