Several beneficial properties have already been observed for mushrooms extracts containing polysaccharides, one of the main components present in these fungi. In order to obtain these extracts and clarify their composition, different extraction and characterization methods can be applied, contributing to understanding its biological effects. In this research, we evaluated three different crude fractions obtained through cold water, hot water, and alkaline solution, from the mushroom Lactarius quieticolor. Each sample was totally characterized by NMR (H, C, HSQC-DEPT, COSY), GC-MS (monosaccharide composition and methylation analysis) and HPSEC (homogeneity and relative Mw). The results showed a mixture of different polysaccharides, including D-galactans and D-glucans in each fraction. All the extracts presented immunostimulant activity in murine RAW 264.7 macrophages, stimulating NO secretion and inducing morphological alterations that were typical of activated cells. These effects were more intense for alkaline fraction (EA), which have higher amounts of mannose and 3,6-di-O-substituted-Glcp units. The results obtained show that crude fractions can potentially stimulate immune cells, which indicates that the consumption of this edible mushroom can bring great benefits to health, being considered an excellent food due to its good biological properties.
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http://dx.doi.org/10.1016/j.foodres.2024.115205 | DOI Listing |
Food Res Int
December 2024
Department of Biochemistry and Molecular Biology, Federal University of Paraná, Curitiba-PR CEP 81531-980, Brazil. Electronic address:
Several beneficial properties have already been observed for mushrooms extracts containing polysaccharides, one of the main components present in these fungi. In order to obtain these extracts and clarify their composition, different extraction and characterization methods can be applied, contributing to understanding its biological effects. In this research, we evaluated three different crude fractions obtained through cold water, hot water, and alkaline solution, from the mushroom Lactarius quieticolor.
View Article and Find Full Text PDFJ Ethnobiol Ethnomed
October 2016
Department of Botany, Institute of Applied Biotechnology and Basic Sciences, University of Rzeszów, Werynia 502, 36-100, Kolbuszowa, Poland.
Background: The study of plants and fungi sold in open-air markets is an important part of ethnobotanical enquiry. Only few such studies were carried out in Europe.
Methods: Four of the largest open-air markets of south-eastern Poland were visited regularly, and the plants sold in them were recorded between 2013 and 2015.
Mycorrhiza
November 2013
University of the Basque Country UPV/EHU, Dpto. Biología Vegetal y Ecología, Apdo.644, 48080, Bilbao, Bizkaia, Spain,
Mycorrhizal inoculation of conifer roots is a key strategy to optimize establishment and performance of forest tree species under both natural and cultivated conditions and also to mitigate transplantation shock. However, despite being a common practice, inoculation in outdoor nursery conditions has been poorly studied. Here, we have evaluated effectiveness of four fungal species (Lactarius deliciosus, Lactarius quieticolor, Pisolithus arhizus, and Suillus luteus) in the production of mycorrhizal Pinus pinaster seedlings in an outdoor commercial nursery and their ability to improve seedling physiology and field performance.
View Article and Find Full Text PDFMycorrhiza
April 2009
Laboratory of Environmental Mycoscience, Division of Environmental Science and Technology, Graduate School of Agriculture, Kyoto University, Kyoto, 606-8502, Japan.
The nitrogen-fixing tree black locust (Robinia pseudoacacia L.) seems to affect ectomycorrhizal (ECM) colonization and disease severity of Japanese black pine (Pinus thunbergii Parl.) seedlings.
View Article and Find Full Text PDFMycorrhiza
May 2007
Department of Forest Mycology and Pathology, Swedish University of Agricultural Sciences, P.O. Box 7026, SE-750 07, Uppsala, Sweden.
In northern forest ecosystems, most soil nitrogen (N) is in organic form and forest trees are largely dependent on ectomycorrhizal (ECM) fungi and their degradative abilities for N uptake. The ability of ECM fungi to acquire N from organic substrates should, therefore, be a widespread trait given its ecological importance. However, little is known about the degradative abilities of most ECM fungi as they remain untested due to problems of isolation or extremely slow growth in pure culture.
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