A novel rod-shaped, Gram-stain-negative bacterial strain MS17 was obtained from a co-culture of Microcystis aeruginosa and Myriophyllum spicatum. The examination of the 16S ribosomal RNA gene sequence showed a significant degree of similarity between strain MS17 and Paucibacter sediminis S2-9 (98.4%), Roseateles violae PFR6 (98.1%), 'Roseateles cellulosilyticus' P8 (98.0%), Roseateles aquae APW11 (97.9%), Roseateles oligotrophus CHU3 (97.7%), Roseateles saccharophilus DSM 654 (97.6%), Kinneretia aquatilis CR182 (97.7%), Pelomonas aquatica CCUG 52575 (97.6%), and Roseateles toxinivorans 2C20 (97.0%). Between strain MS17 and the type strains of closely related species, digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values were below 21.9% and 78.48%. The respiratory quinone was ubiquinone Q-8. The main fatty acids (> 10.0%) were C ω6c, C, C, and summed feature 3 (C ω7c and/or C ω6c). The polar lipids comprised phosphatidylethanolamine, unidentified phospholipid, phosphatidylglycerol, unidentified aminophospholipid, unidentified aminolipid, and three unidentified lipids. The genomic G + C content was 65.9%. According to phenotypic characteristics, phylogenetic relationships, and chemotaxonomic data, strain MS17 has been categorized as a newly discovered species belonging to the Roseateles genus, and the name Roseateles microcysteis sp. nov. is suggested. It exhibits distinct biochemical properties that differentiate it from closely related species. The type strain is MS17 (=KCTC 8001 = LMG 33142).
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/s00284-024-04002-2 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Roseateles microcysteis sp. nov., Isolated from Co-cultivation of Microcystis aeruginosa and Myriophyllum spicatum.
Curr Microbiol
November 2024
Cell Factory Research Center, Korea Research Institute of Bioscience and Biotechnology, 125 Gwahak-ro, Yuseong-gu, Daejeon, 34141, Republic of Korea.
A novel rod-shaped, Gram-stain-negative bacterial strain MS17 was obtained from a co-culture of Microcystis aeruginosa and Myriophyllum spicatum. The examination of the 16S ribosomal RNA gene sequence showed a significant degree of similarity between strain MS17 and Paucibacter sediminis S2-9 (98.4%), Roseateles violae PFR6 (98.
View Article and Find Full Text PDFThe AMP-Activated Protein Kinase (AMPK) Positively Regulates Lysine Biosynthesis Induced by Citric Acid in .
J Fungi (Basel)
March 2023
Sanya Institute of Nanjing Agricultural University, Key Laboratory of Agricultural Environmental Microbiology Ministry of Agriculture, Department of Microbiology, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China.
, the most produced edible mushroom species in China, is rich in lysine. Further enhancing its lysine biosynthesis is vital for improving its quality in industrialized cultivation. Citric acid induction significantly increases both the biomass and growth rate of hyphae, as well as the lysine content.
View Article and Find Full Text PDFHeme Oxygenase/Carbon Monoxide Participates in the Regulation of Heat-Stress Response, Ganoderic Acid Biosynthesis, and Cell-Wall Integrity.
Int J Mol Sci
October 2022
Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Department of Microbiology, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China.
Carbon monoxide (CO), a product of organic oxidation processes, arises in vivo principally from the enzymatic reaction of heme oxygenase (HO, transcription gene named ). HO/CO has been found to exert many salutary effects in multiple biological processes, including the stress response. However, whether HO/CO is involved in the regulation of the heat-stress (HS) response of () is still poorly understood.
View Article and Find Full Text PDFEstablishment of CRISPR/Cas9 Genome-Editing System Based on Dual sgRNAs in .
J Fungi (Basel)
June 2022
Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Department of Microbiology, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China.
Article Synopsis
- The text discusses the use of CRISPR/Cas9 technology for precision genome editing of a commercially important edible fungus, previously referred to as "Asian."
- The authors successfully developed a genome-editing system using plasmids containing codon-optimized Cas9 and dual sgRNAs, resulting in mutants that show resistance to 5-fluorooric acid (5-FOA).
- The study identified a native U6 promoter and demonstrated heritable genomic changes in the mutants, marking the first successful CRISPR/Cas9 application in this type of fungus, thus expanding its potential for molecular breeding.
Chitosan Increases Lysine Content through Amino Acid Transporters in .
Foods
July 2022
Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Department of Microbiology, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China.
Article Synopsis
- - The study aimed to enhance lysine content (a vital quality indicator) using chitosan, determining optimal conditions of 14.61 μg/mL concentration and 52.90 hours treatment, resulting in a theoretical lysine value of 30.95 mg/g.
- - Genetic analysis identified 11 members of amino acid transporter (AAT) genes, with AAT3 and AAT4 showing significant expression increases after chitosan treatment and impacting lysine biosynthesis regulation.
- - Chitosan treatment led to a 26.41% increase in lysine in the wild-type strain, while silencing AAT3 and AAT4 genes caused a decrease in lysine content, suggesting AAT3
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!