Insights into antibiotic resistance promoted by quinolone exposure.

Antimicrob Agents Chemother

Department of Biological Chemistry, John Innes Centre, Norwich Research Park, Norwich, United Kingdom.

Published: November 2024

AI Article Synopsis

Article Abstract

Quinolone-induced antibiotic resistance (QIAR) refers to the phenomenon by which bacteria exposed to sublethal levels of quinolones acquire resistance to non-quinolone antibiotics. We have explored this in MG1655 using a variety of compounds and bacteria carrying a quinolone-resistance mutation in gyrase, mutations affecting the SOS response, and mutations in error-prone polymerases. The nature of the antibiotic-resistance mutations was determined by whole-genome sequencing. Exposure to low levels of most quinolones tested led to mutations conferring resistance to chloramphenicol, ampicillin, kanamycin, and tetracycline. The mutations included point mutations and deletions and could mostly be correlated with the resistance phenotype. QIAR depended upon DNA gyrase and involved the SOS response but was not dependent on error-prone polymerases. Only moxifloxacin, among the quinolones tested, did not display a significant QIAR effect. We speculate that the lack of QIAR with moxifloxacin may be attributable to it acting via a different mechanism. In addition to the concerns about antimicrobial resistance to quinolones and other compounds, QIAR presents an additional challenge in relation to the usage of quinolone antibacterials.

Download full-text PDF

Source
http://dx.doi.org/10.1128/aac.00997-24DOI Listing

Publication Analysis

Top Keywords

antibiotic resistance
8
levels quinolones
8
sos response
8
error-prone polymerases
8
quinolones tested
8
resistance
6
mutations
6
qiar
5
insights antibiotic
4
resistance promoted
4

Similar Publications

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!