Phytochemical Characterization and Comparative Analysis of Cycloartane-Type Triterpenes in Astragalus adsurgens and Astragalus membranaceus.

, a significant forage plant cultivated in arid regions of northwest China, remains underexplored for its triterpenoid saponins and medicinal properties compared to the extensively studied . To explore the phytochemical profile of for its potential application in the medical field, we employed ultra-pressure liquid chromatography coupled with a tandem mass spectrometry-based method to identify cycloartane-type triterpenes. Eventually, five new cycloartane-type triterpenoids, adsurgosides A - D ( 1 - 4: ) and 3-methyl-3,4-seco-cyclostellanol (5: ), together with two known analogs, cycloastragenol (6: ) and cyclopycanthogenin (7: ), were isolated from the roots of . Their structures were elucidated using 1D and 2D NMR analyses in combination with HRESIMS data. Additionally, a comparative study on the distribution patterns of these compounds revealed qualitative and quantitative variations between and . Our findings not only identified an alternative plant for isolating cycloartane-type triterpenoids but also offer new insights into the chemical properties of .