This study aimed to clarify the effect of the accuracy of phantom-making on quantification accuracy in the measurement of system planar sensitivity using a disc source. We measured the system planar sensitivity using the method recommended by the equipment manufacturer. A container ofTc solution was placed at a height of 10 cm from the surface of the collimator using styrofoam. The system planar sensitivity was the average of the values obtained from the two detectors, the measurements were taken thrice. The system planar sensitivity values obtained when the disc source size (5.0, 14.5 and 20 cm), solution volume (50, 100 and 150 ml) and radioactivity (nine points between 36 MBq and 505 MBq) changed were used to evaluate the quantification accuracy. The relative error for the 5.0 cm source significantly exceeded for the other source sizes (p < 0.05); however, no statistically significant difference was observed between the 14.5 cm and 20.0 cm sources. The smaller the solution volume, the smaller the relative error and the higher the quantification accuracy. The quantification accuracy increased as radioactivity decreased. The relative error was lowest at 36 MBq and highest at 505 MBq, with a difference of approximately 10%. Quantification accuracy improved as the size of the surface source exceeded 14.5 cm and the amount of solution decreased. Radioactivity at the time of measurement significantly affected quantification accuracy and 73-103 MBq of radioactivity was sufficient for repeatable and quantification accuracy.
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http://dx.doi.org/10.1088/2057-1976/ad91bc | DOI Listing |
Bioanalysis
January 2025
Bioanalysis Discovery & Development Sciences, Johnson & Johnson, Spring House, PA, USA.
Background: Most oligonucleotide bioanalytical assays currently only quantify the pharmacologically-active antisense strand, though there have been recent efforts to simultaneously quantify the sense strand using hybridization ELISA or solid phase extraction LC-MS. Hybrid LC-MS, which offers both high sensitivity and specificity unlike the currently used platforms, has not been applied to quantify both siRNA strands simultaneously.
Materials & Methods: A hybrid LC-MS assay utilizing LNA capture probes was developed and applied to quantify both strands of a 21-mer lipid-conjugated siRNA (SIR-3) using tandem mass spectrometry (MS/MS).
Cureus
December 2024
Emergency, Hospital de Braga, Braga, PRT.
Pericardial effusion refers to the accumulation of fluid within the pericardial sac, the double-layered membrane surrounding the heart. It can be caused by various medical conditions and may lead to serious complications if not diagnosed and managed promptly. Point-of-care ultrasound (POCUS) has emerged as a valuable tool in the clinical evaluation of pericardial effusions, offering real-time visualization and aiding in the assessment of its size, characteristics, and potential hemodynamic impact.
View Article and Find Full Text PDFSemin Pediatr Surg
January 2025
Section of Pediatric Surgery, Pediatric Liver and Gut Research Group, New Children's Hospital, University of Helsinki and Helsinki University Hospital, Helsinki, Finland; Pediatric Research Center, Children's Hospital, University of Helsinki and Helsinki University Hospital, Helsinki, Finland; Department of Women's and Children's Health, Karolinska Institute, Stockholm, Sweden. Electronic address:
Regardless of the underlying etiology and success of PE, progressive liver fibrosis and eventually cirrhosis represent the dominant pathology and the end-stage of BA. Ascending bile duct injury-induced cholestasis, inflammation and ductular reaction provide profibrogenic cytokine environment leading to myofibroblast activation and rapid progression of fibrosis especially after unsuccessful portoenterostomy. Although liver fibrosis and development of cirrhosis play a crucial role in determining BA outcomes, the exact prognostic significance and dynamics of mild to moderate liver fibrosis remain unclear.
View Article and Find Full Text PDFTalanta
January 2025
Ampere - Laboratório de Plataformas Eletroquímicas. Departamento de Química, Universidade Federal de Santa Catarina, 880400-900, Florianópolis, SC, Brazil. Electronic address:
Nicotine (NIC) detection is vital for monitoring its presence in various environments, including tobacco products, electronic cigarettes, and clinical samples; NIC's widespread use and health implications necessitate precise and reliable detection methods as it is linked to diseases such as lung cancer and vascular disorders. In this study, we developed and characterized Au tadpole-like nanostructures immobilized onto titanium oxide (TiO) to provide a cost-effective and sensitive NIC detection material. The comprehensive characterization of the composite used transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), and X-ray diffraction (XRD), showing the robustness of the synthesis.
View Article and Find Full Text PDFComput Biol Med
January 2025
Department of Bioengineering, Imperial College London, London SW7 2AZ, UK. Electronic address:
Temporal echocardiography image registration is important for cardiac motion estimation, myocardial strain assessments, and stroke volume quantifications. Deep learning image registration (DLIR) is a promising way to achieve consistent and accurate registration results with low computational time. DLIR seeks the image deformation that enables the moving image to be warped to match the fixed image.
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