Transcription- and Replication-Competent Virus-like Particle Systems for Marburg Virus.

Methods Mol Biol

Department of Virology, Immunology & Microbiology, Chobanian & Avedisian School of Medicine and National Emerging Infectious Diseases Laboratories, Boston University, Boston, MA, USA.

Published: November 2024

Here, we describe the transcription- and replication-competent virus-like particle (trVLP) system for Marburg virus (MARV), which recapitulates transcription and replication of the viral genome in addition to viral particle assembly, egress, and entry. This protocol includes instructions for transfections for producer and acceptor cells and the use of trVLPs for infection.

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Source
http://dx.doi.org/10.1007/978-1-0716-4256-6_10DOI Listing

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