Marburg Virus Minigenome Assays.

Methods Mol Biol

Department of Virology, Immunology and Microbiology, Chobanian & Avedisian School of Medicine and National Emerging Infectious Diseases Laboratories, Boston University, Boston, MA, USA.

Published: November 2024

This chapter describes minigenome systems for Marburg virus (MARV), which reconstitute the viral polymerase complex functions of gene expression and genome replication. Procedures covered herein include passage and seeding of cells, transfection, sample collection, and reporter gene assays.

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http://dx.doi.org/10.1007/978-1-0716-4256-6_9DOI Listing

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