To date, the only probiotic yeast with evidence of health-promoting effects is var. . The expanded market including dietary supplements and functional foods supplemented with var. creates an environment conductive to food adulterations, necessitating rapid testing to verify product probiotic status. Herein, qPCR-HRM analysis was tested for probiotic yeast identification. The effectiveness of the primer pairs' set was examined, designed to amplify heterogeneous regions in (a) rDNA sequences previously designed to identify food-derived yeast and (b) genes associated with physiological and genotypic divergence of var. Preliminary tests of amplicons' differentiation power enabled the selection of interspecies sequences for and ITS and genus-specific sequences , , and The multi-fragment qPCR-HRM analysis was sufficient for culture-dependent var. identification and proved effective in the authentication of dietary supplements' probiotic composition. The identification of var. in complex microbial mixtures of kefir succeeded with more specific intragenus sequences and The predominance of var. in the tested matrices, quantitatively corresponded to the probiotic-enriched food, was crucial for identification with qPCR-HRM analysis. Considering the reported assumptions, qPCR-HRM analysis is an appropriate tool for verifying probiotic-enriched food.
Download full-text PDF |
Source |
---|---|
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11586983 | PMC |
http://dx.doi.org/10.3390/biotech13040048 | DOI Listing |
Pathogens
October 2024
Division of Parasitology and Parasitic Diseases, Department of Preclinical Sciences, Institute of Veterinary Medicine, Warsaw University of Life Sciences, 02-786 Warsaw, Poland.
The nematode is responsible for a vector-borne disease affecting canines and humans worldwide, known as cardiopulmonary dirofilariasis. An accurate and early diagnosis is of the utmost importance for effective disease management. While traditional microscopy-based methods remain invaluable, they have inherent limitations.
View Article and Find Full Text PDFBioTech (Basel)
November 2024
Department of Biotechnology and Food Microbiology, Poznan University of Life Sciences, Wojska Polskiego 48, 60-637 Poznan, Poland.
To date, the only probiotic yeast with evidence of health-promoting effects is var. . The expanded market including dietary supplements and functional foods supplemented with var.
View Article and Find Full Text PDFMed Vet Entomol
November 2024
Department of Epidemiology and Public Health, Veterinary Institute, Federal Rural University of Rio de Janeiro, Seropédica, Rio de Janeiro, Brazil.
Culex species, such as Culex quinquefasciatus and Culex nigripalpus display a range of feeding habits and act as vectors for pathogens that can cause diseases in both humans and animals. Understanding their feeding habits is pivotal for enhancing disease prevention strategies. The present study introduces the application of two multiplex real-time PCR (qPCR) followed by high-resolution melting (HRM) as a cost-effective and time-efficient alternative.
View Article and Find Full Text PDFPoult Sci
December 2024
Institute of Animal Husbandry and Veterinary Medicine/Fujian Key Laboratory for Avian Diseases Control and Prevention/Fujian Animal Diseases Control Technology Development Centre, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China. Electronic address:
Plant Dis
September 2024
Universidad de Chile, Avenida Santa Rosa 11.315, Santiago, Chile, 882-0808;
The gray mold (Botrytis cinerea; Botrytis) is the main disease affecting grapevines production in Chile. Succinate Dehydrogenase Inhibitors (SDHI) belonging to the carboxamides fungicide family are a key tool for the control of Botrytis in grapevines from Chilean Central Valley. This study aimed to determine the sensitivity of Chilean Botrytis population to the new generation carboxamide pydiflumetofen.
View Article and Find Full Text PDFEnter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!