Development and evaluation of RNA microsphere-based RNAi approaches for managing the striped flea beetle (Phyllotreta striolata), a globally destructive pest of Cruciferae crops.

Background: RNA interference (RNAi) technology has emerged as a promising strategy for species-specific management of agricultural pests. However, the application of this technology has been significantly hindered by the instability of the interfering RNA molecules in the insect body after ingestion leading to variations in the susceptibility to the RNA triggers across different taxonomic groups of insects. Therefore, it is necessary to develop new approaches that will overcome these challenges associated with the use of RNAi-based insect pest management strategies. This study explored the use of RNA microspheres (RMS) synthesized via rolling-circle transcription (RCT) technology as a potential method for managing striped flea beetle (Phyllotreta striolata), a globally destructive pest of Cruciferae crops.

Results: The synthesized RMS against the genes encoding reticulocalbin (RMS-PsRCN) and ribosomal RNA (RMS-PsrRNA) were highly effective in both silencing their target genes and causing increased P. striolata adult mortality. Relative expression levels of the target genes RMS-PsRCN and RMS-PsrRNA were decreased by 74.9% and 68.92%, respectively, in RMS fed adults, compared with the control adults fed RMS-EGFP. Consequently, the adult mortalities were 81.7% and 73.3% when fed RMS-PsRCN and RMS-PsrRNA, respectively, compared with 8.3% in the control adults. Furthermore, movements of adults fed RMS-PsRCN and RMS-PsrRNA were decreased by 70.2% and 55.7%, respectively, compared with the control adults.

Conclusions: This study shows the potential of using RMS to suppress the expression of target genes and subsequently produce significant mortality rates and behavioral changes in RMS-fed adult P. striolata. These findings underscore the promises and viability of using RMS as an effective strategy for gene function studies and species-specific management of agricultural important insect pests. © 2024 Society of Chemical Industry.