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[Quantitative comparison of phospho-proteins of at different growing phases]. | LitMetric
Protein phosphorylation plays a key role in , the pathogen of tuberculosis, holding promise as a new target of anti-tuberculosis drugs. We used . , a close relative of . , as a model organism to study the protein phosphorylation at different growth phases. We identified 573 phosphorylated peptides and 816 phosphorylated sites of 385 proteins in the . samples at both logarithmic and stationary phases, and then established a comprehensive dataset of phosphorylated proteins in . . By comparing the expression levels of phosphorylated proteins between the logarithmic and the stationary phase with the selected ion monitoring (SIM) strategy, we verified 68 upregulated proteins involved in cell division and protein translation, and 69 downregulated proteins mainly involved in the tricarboxylic acid cycle pathway. The differentially expressed phosphorylated proteins were significantly enriched in important cellular cycle events such as cell elongation and division. The findings of this study provide proteome evidence for elucidating the phosphorylation in both . and . .
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