AI Article Synopsis
- The integration of large fluorescent protein tags by CRISPR can be inefficient, prompting the optimization of split-GFP and split-Scarlet systems for better results.
- Initial research showed successful gene expression without effects on reproduction, but recent findings indicate that one specific transgene may cause sterility after prolonged culturing at 25.5 °C.
- These results highlight potential issues with using certain fusion proteins, urging caution in their application.
Article Abstract
The integration of fluorescent protein tags (GFP, mScarlet, mNeon, etc) by CRISPR is often inefficient due to the size of the tags (hundreds of base pairs). To facilitate fluorescent tagging, the split-GFP and split-Scarlet systems have been optimized for use in (Goudeau et al., 2021). In , allows germline gene expression of red fusion proteins. While initial studies reported wild-type broods at both permissive and non-permissive temperatures, we report here that at least one such transgene confers sterility after continuous culturing at 25.5 °C. This serves as a cautionary tale for use of the driver and/or T2A::mScarlet fusions to this protein.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11584908 | PMC |
| http://dx.doi.org/10.17912/micropub.biology.001343 | DOI Listing |
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