is Involved in, but not the Primary Cause of Creeping Bentgrass Decline of Putting Greens in Southern California.

Root-knot nematodes were discovered in severely declining creeping bentgrass putting greens at a golf course in Indian Wells, Riverside County, California. The exhibited disease symptoms included chlorosis, stunted growth, and dieback. Based on morphological examination and measurements of J2 females and males, it was suggested that the causal pathogen was . This identification was confirmed by analysis of the D2-D3 expansion segments of 28S rRNA and gene sequences. The host status of 28 plant species was evaluated in greenhouse trials. All tested monocots, except rye and species, were found to be hosts, while no reproduction occurred on dicots. Temperature-tank experiments helped determine that the life cycle of was completed between 17-35 °C, with a base temperature of 8.3 °C and a required heat sum of 493 degree-days (DD). In greenhouse trials in pasteurized soil and near-ideal growing conditions, did not cause significant growth reduction of creeping bentgrass cv. Penn A-4, even at very high J2 inoculation densities. It is highly probable that other biotic and abiotic factors contributed to the observed putting green damage.