AI Article Synopsis
- The study focuses on a wild tuber-bearing species from the Solanaceae family and successfully sequenced its chloroplast genome using Illumina technology.
- The complete chloroplast genome measures 155,547 base pairs, featuring distinct regions including a large single copy, a small single copy, and two inverted repeat regions, with a total of 158 functional genes identified.
- Phylogenetic analysis shows that this species is closely related to cultivated potatoes, offering valuable genomic insights for future breeding and evolutionary research.
Article Abstract
is one of the wild tuber-bearing species belonging to the Solanaceae family. In this study, the chloroplast genome sequence of the species was completed with Illumina sequencing technology. The total length of the chloroplast genome is 155,547 bp with a GC content of 37.87%. It comprises a large single copy (LSC) region of 85,941 bp, a small single copy (SSC) region of 18,382 bp, and two inverted repeat regions (IRa and IRb) of 25,612 bp. Additionally, 158 functional genes in the genome were identified, including 105 protein-coding genes, eight ribosomal RNA genes, and 45 transfer RNA genes. Phylogenetic analysis revealed that is grouped into a large clade with other species including cultivated potatoes (). This study provides useful genomic information for future breeding and evolutionary studies of and other species.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11583358 | PMC |
| http://dx.doi.org/10.1080/23802359.2024.2432357 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Cloning a Chloroplast Genome in and .
Bio Protoc
January 2025
Biochemistry Department, Western University, London, Canada.
Chloroplast genomes present an alternative strategy for large-scale engineering of photosynthetic eukaryotes. Prior to our work, the chloroplast genomes of (204 kb) and (140 kb) had been cloned using bacterial and yeast artificial chromosome (BAC/YAC) libraries, respectively. These methods lack design flexibility as they are reliant upon the random capture of genomic fragments during BAC/YAC library creation; additionally, both demonstrated a low efficiency (≤ 10%) for correct assembly of the genome in yeast.
View Article and Find Full Text PDFMoCloro: an extension of the Chlamydomonas reinhardtii modular cloning toolkit for microalgal chloroplast engineering.
Physiol Plant
January 2025
Laboratory of Biochemistry, Institut Químic de Sarrià, Universitat Ramon Llull, Barcelona, Spain.
Photosynthetic microalgae are promising green cell factories for the sustainable production of high-value chemicals and biopharmaceuticals. The chloroplast organelle is being developed as a chassis for synthetic biology as it contains its own genome (the plastome) and some interesting advantages, such as high recombinant protein titers and a diverse and dynamic metabolism. However, chloroplast engineering is currently hampered by the lack of standardized cloning tools and Design-Build-Test-Learn workflows to ease genomic and metabolic engineering.
View Article and Find Full Text PDFPagHCF106 negatively regulates drought stress tolerance in poplar (Populus alba × Populus glandulosa) by modulating stomatal aperture.
Tree Physiol
January 2025
Institute of Soil and Water Conservation, Northwest A&F University, Yangling 712100, China.
Modulation of stomatal development and movement is a promising approach for creating water-conserving plants. Here, we identified and characterized the PagHCF106 gene of poplar (Populus alba × Populus glandulosa). The PagHCF106 protein localized predominantly to the chloroplast, and the PagHCF106 gene exhibited tissue-specific expression pattern.
View Article and Find Full Text PDFIntraspecific identification and genetic diversity analysis of Paeonia lactiflora based on chloroplast genes rpoB and psbK-psbI.
Mol Biol Rep
January 2025
School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, 102488, People's Republic of China.
Background: Paeonia lactiflora Pall., a member of Paeoniaceae family, is a medicinal herb widely used in traditional Chinese medicine. Chloroplasts are multifunctional organelles containing distinct genetic material.
View Article and Find Full Text PDFStructural characterization and phylogenetic analysis of the chloroplast genome of var. balansae (gagnep.) N. Jacobsen 1991.
Mitochondrial DNA B Resour
January 2025
Department of Ecology and Institute of Hydrobiology, Jinan University, Guangzhou, China.
is a popular ornamental aquatic plant for aquarists, although only six species are found in China. Destruction of the natural habitats of for human activities has led to a decline in its numbers. In this report, we sequenced and annotated the chloroplast genome for the first time.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!