Detection of microorganisms from bovine using real-time PCR (Dembo PCR) is a comprehensive detection technique that was developed to detect pathogens causing bovine diseases. In Japan, the definitive tests for monitored infectious diseases, which are defined by law, are carried out at government agencies. On the other hand, the existence of pathogens other than monitored infectious diseases are not well understood. From the perspective of livestock quarantine, it is important to make it possible to identify pathogens other than monitored infectious diseases, so we extended the existing Dembo PCR system in this study. In particular, the number of targets other than monitored infectious disease was increased. The new version of Dembo PCR may be useful in elucidating new pathologies associated with multiple pathogens.
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Development of Dembo PCR for cattle in accordance with Japanese livestock quarantine guidelines.
J Vet Med Sci
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Center for Infectious Disease Epidemiology and Prevention Research, Tokyo University of Agriculture and Technology.
Detection of microorganisms from bovine using real-time PCR (Dembo PCR) is a comprehensive detection technique that was developed to detect pathogens causing bovine diseases. In Japan, the definitive tests for monitored infectious diseases, which are defined by law, are carried out at government agencies. On the other hand, the existence of pathogens other than monitored infectious diseases are not well understood.
View Article and Find Full Text PDFDembo polymerase chain reaction technique for detection of bovine abortion, diarrhea, and respiratory disease complex infectious agents in potential vectors and reservoirs.
J Vet Sci
May 2018
Research and Education Center for Prevention of Global Infectious Diseases of Animals, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Tokyo 183-0045, Japan.
Bovine abortion, diarrhea, and respiratory disease complexes, caused by infectious agents, result in high and significant economic losses for the cattle industry. These pathogens are likely transmitted by various vectors and reservoirs including insects, birds, and rodents. However, experimental data supporting this possibility are scarce.
View Article and Find Full Text PDFDevelopment of a one-run real-time PCR detection system for pathogens associated with bovine respiratory disease complex.
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Research and Education Center for Prevention of Global Infectious Diseases of Animals, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai, Fuchu, Tokyo 183-8509, Japan.
Bovine respiratory disease complex (BRDC) is frequently found in cattle worldwide. The etiology of BRDC is complicated by infections with multiple pathogens, making identification of the causal pathogen difficult. Here, we developed a detection system by applying TaqMan real-time PCR (Dembo respiratory-PCR) to screen a broad range of microbes associated with BRDC in a single run.
View Article and Find Full Text PDFDevelopment of a novel detection system for microbes from bovine diarrhea by real-time PCR.
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The United Graduate School of Veterinary Sciences, Gifu University, Yanagito, Gifu 501-1193, Japan.
Diarrhea in cattle is one of the most economically costly disorders, decreasing milk production and weight gain. In the present study, we established a novel simultaneous detection system using TaqMan real-time PCR designed as a system for detection of microbes from bovine diarrhea using real-time PCR (referred to as Dembo-PCR). Dembo-PCR simultaneously detects a total of 19 diarrhea-causing pathogens, including viruses, bacteria and protozoa.
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