AI Article Synopsis
- The study identifies four sucrose transporter genes (AcSUTs) in pineapple, highlighting their roles in plant growth and response to cold stress.
- Two specific transporters (AcSUT1A and AcSUT1B) were shown to effectively transport glucosides and had a strong affinity for sucrose, indicating their importance in nutrient uptake.
- The research revealed that overexpressing AcSUT1B enhanced cold tolerance in transgenic Arabidopsis and established a link between the AcCBF1 protein and the activation of AcSUT1B under cold conditions, advancing our understanding of cold response mechanisms in pineapple.
Article Abstract
Sucrose transporter (SUT) plays essential roles in plant growth and development, as well as responses to diverse abiotic stresses. However, limited information about the function of SUT was available in pineapple, an important tropical fruit crop with crassulacean acid metabolism. Here, four AcSUT genes were identified in pineapple genome, and divided into three clades according to the phylogenetic analysis. The expression profiles of AcSUTs were systemically examined, and they were all localized to plasma membrane. Transport activity assay by two-electrode voltage clamp of Xenopus oocytes showed that AcSUT1A and AcSUT1B were capable of transporting a range of glucosides, and they were exhibited high affinity for sucrose with Km value of 0.09 mM and 0.41 mM at pH 5.0, respectively. Overexpression of the cold-induced AcSUT1B conferred enhanced cold tolerance in transgenic Arabidopsis. DNA-protein interaction analysis further demonstrated that AcCBF1 directly binds the CRT/DRE element of the AcSUT1B promoter and activated its expression. Heterologous expression of AcCBF1 in Arabidopsis also increased cold tolerance. In this study, we investigated the transport activities of AcSUTs in pineapple and identified the AcCBF1-AcSUT1B module involved in cold stress, which provided new insights into the molecular mechanism of the cold response in pineapple.
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| http://dx.doi.org/10.1016/j.ijbiomac.2024.137952 | DOI Listing |
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