AI Article Synopsis
- The study investigates the role of TRIM71 as a potential therapeutic target for laryngeal squamous cell carcinoma (LSCC), a common head and neck tumor, which has been on the rise.
- TRIM71 was found to be significantly downregulated in LSCC tissues, and its overexpression inhibited cell growth and tumor development in xenograft models.
- The research suggests that TRIM71 may exert its anti-tumor effects by regulating the stability of eIF5A2 protein, indicating that targeting TRIM71 could be a promising strategy for LSCC treatment.
Article Abstract
The incidence of laryngeal squamous cell carcinoma (LSCC) has been rising recently. LSCC is one of the most prevalent malignant tumors of the head and neck. In this study, we aimed to investigate whether tripartite motif containing 71 (TRIM71) could serve as a molecular target for the treatment of LSCC. The mRNA and protein levels were examined by using real-time qPCR and Western blot, respectively. Cell proliferation was determined by cell-counting kit 8 assay. To further confirm the function of TRIM71 in LSCC, an in vivo cell line-derived xenograft study was conducted. The half-life of eukaryotic translation initiation factor 5A2 (eIF5A2) protein was measured by cycloheximide chase assay. Our results showed that TRIM71 was significantly downregulated in LSCC tumor tissues. TRIM71 overexpression significantly inhibited LSCC cell growth and suppressed tumor volume and weight in the xenograft models. The interaction between TRIM71 and eIF5A2 was verified by co-immunoprecipitation assay. Moreover, overexpression of TRIM71 in LSCC cells significantly inhibited the protein expression of eIF5A2 by down-regulating its stability, while it did not affect its mRNA level. In contrast, overexpression of eIF5A2 abolished the anti-tumor effects of TRIM71. In summary, TRIM71 may exert its anti-tumor effects through regulating eIF5A2, highlighting the potential of TRIM71 as an effective therapeutic target for the treatment of LSCC.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/s12010-024-05084-1 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
DYRK4 upregulates antiviral innate immunity by promoting IRF3 activation.
EMBO Rep
December 2024
Taikang Medical School (School of Basic Medical Sciences), Wuhan University, 430071, Wuhan, China.
Viral infection activates the transcription factors IRF3 and NF-κB, which induce type I interferon (IFN) and antiviral innate immune responses. Here, we identify dual-specific tyrosine phosphorylation-regulated kinase 4 (DYRK4) as an important regulator of virus-triggered IFN-β induction and antiviral innate immunity. Overexpression of DYRK4 enhances virus-triggered activation of IRF3 and type I IFN induction, whereas knockdown or knockout of DYRK4 impairs virus-induced activation of IRF3 and NF-κB.
View Article and Find Full Text PDFTripartite Motif Containing 71 Suppresses Tumor Growth by Down-Regulating eIF5A2 Expression in Laryngeal Squamous Cell Carcinoma.
Appl Biochem Biotechnol
November 2024
Department of Otolaryngology, the Second Hospital of Hebei Medical University, No. 215 Heping West Road, Shijiazhuang, 050000, Hebei, China.
Article Synopsis
- The study investigates the role of TRIM71 as a potential therapeutic target for laryngeal squamous cell carcinoma (LSCC), a common head and neck tumor, which has been on the rise.
- TRIM71 was found to be significantly downregulated in LSCC tissues, and its overexpression inhibited cell growth and tumor development in xenograft models.
- The research suggests that TRIM71 may exert its anti-tumor effects by regulating the stability of eIF5A2 protein, indicating that targeting TRIM71 could be a promising strategy for LSCC treatment.
Mitochondrial E3 ligase TRIM71 affects mitochondrial complex assembly and sensitizes dopaminergic neuronal cells to apoptosis in Parkinson's Disease (PD).
Int J Biochem Cell Biol
December 2024
Department of Biochemistry, Faculty of Science, The M.S. University of Baroda, Vadodara, Gujarat 390 002, India. Electronic address:
Article Synopsis
- Parkinson's Disease (PD) is a progressive disorder affecting motor and non-motor functions, rooted in mitochondrial dysfunction leading to loss of dopaminergic neurons.
- An E3 ubiquitin ligase, TRIM71, has been studied for its role in mitochondrial health and its connection to neuronal death under PD conditions, specifically in models exposed to toxins like rotenone and 6-OHDA.
- The study found that TRIM71 promotes neuronal death in PD stress, as its increased presence on mitochondria adversely affects mitochondrial function and increases oxidative stress; whereas reducing TRIM71 levels improves mitochondrial health and cell survival.
Oncofetal TRIM71 drives liver cancer carcinogenesis through remodeling CEBPA-mediated serine/glycine metabolism.
Theranostics
September 2024
Department of Integrative Oncology, Fudan University Shanghai Cancer Center, and Shanghai Key Laboratory of Medical Epigenetics, International Co-laboratory of Medical Epigenetics and Metabolism, Institutes of Biomedical Sciences, Shanghai Medical College, Fudan University, Shanghai, China.
Article Synopsis
- Tumor cells adapt their gene expression to create an environment that supports their growth and aggressive traits, but the key regulatory factors behind this process remain unclear.
- Researchers utilized single-cell RNA sequencing and animal models to investigate liver cancer, discovering that liver tumors reorganize an oncofetal ecosystem to retain malignant characteristics.
- A specific RNA-binding protein called TRIM71 was found to be overexpressed in liver cancers and linked to poor patient outcomes, and targeting TRIM71 with certain treatments showed potential for inhibiting cancer cell growth.
Whole-genome sequencing identifies functional genes for environmental adaptation in Chinese sheep.
J Genet Genomics
November 2024
State Key Laboratory of Animal Biotech Breeding, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100193, China; National Germplasm Center of Domestic Animal Resources, Ministry of Technology, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100193, China. Electronic address:
Article Synopsis
- - The study focuses on the genetic adaptability of 266 sheep from 18 different populations in China, exploring how they have evolved in response to various environmental conditions.
- - Researchers performed whole-genome sequencing and analyzed integration of 131 environmental factors with genetic variations to identify 35 key genes related to features like wool, fat tail formation, reproduction, and growth.
- - A notable finding includes a link between mutations in the PAPSS2 and RXFP2 genes and changes in altitude, highlighting the complex genetic factors that influence sheep adaptation to diverse environments.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!