Fluorescent core-shell SiO@COF composite for ultra-sensitive detection of cysteine and homocysteine.

Mikrochim Acta

College of Chemistry and Material, Jiangxi Normal University, 99 Ziyang Road, Nanchang, 330022, PR China.

Published: November 2024

AI Article Synopsis

  • A core-shell SiO@COF composite was created to enhance the fluorescence properties of covalent organic frameworks (COFs) using SiO as the substrate and specific precursors to form the COF layer.
  • The SiO@COF composite exhibited better dispersion and improved fluorescence compared to pure COF, enabling a responsive "ON-OFF-ON" fluorescence sensing system for detecting cysteine (Cys) and homocysteine (Hcy).
  • With high sensitivity and selectivity, the probe detected Cys and Hcy across precise concentration ranges and showed effective performance in real sample tests, indicating its potential for practical applications.

Article Abstract

To improve the fluorescence properties of covalent organic framework (COF) materials, core-shell SiO@COF composite was prepared, in which SiO was used as inner substrate, and BMTH (2,5-bis (2-methoxyethoxy) p-phenylhydrazide) and HB (2-hydroxy-1,3,5-phenyltrialdehyde) acted as precursors to construct COF layer on the surface of SiO. Compared with COF, SiO@COF composite showed better dispersion property and improved fluorescence performance under the same experimental conditions. Using SiO@COF as a fluorescence probe, the fluorescence intensity was gradually decreased with the addition of Cu, while it was restored by introducing Cys or Hcy, realizing "ON-OFF-ON" fluorescence sensing detection. Other amino acids with five times the concentration of Cys showed little effect on the determination of Cys or Hcy. The SiO@COF-Cu probe exhibited ultrahigh sensitivity and good selectivity for the detection of Cys and Hcy, with a detection range of 0.23 to 250.0 µM for Cys and 0.31 to 170.0 µM for Hcy. The detection limits (LOD = 3 σ/s) were 75 and 104 nM for Cys and Hcy, respectively. The system also showed a good recovery and low relative standard deviations in actual sample tests for Cys or Hcy, demonstrating its potential for practical applications.

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00604-024-06827-xDOI Listing

Publication Analysis

Top Keywords

cys hcy
20
core-shell sio@cof
8
sio@cof composite
8
hcy detection
8
cys
7
hcy
6
detection
5
fluorescent core-shell
4
composite ultra-sensitive
4
ultra-sensitive detection
4

Similar Publications

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!